LOCUS       sequence-87061-e        1214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..759
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             790..1167
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gatcaaggag
       61 aacatgcaca tgaagctgta catggaaggc accgtgaaca accaccactt caagtgcacc
      121 accgaagggg agggcaagcc ctacgagggc acccagaccc agaggattaa ggtggtggag
      181 ggaggccccc tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag
      241 accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac
      361 accagcctcc aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaccac cgagaccctg
      481 taccccgctg acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg
      541 ggccacctgc actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc
      601 aagatgcccg gcgtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat
      661 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca aacttaatgg catggacgag ctgtacaagt ccggactcag atctggaggc
      781 tccggaggcc cagagccagc gaagtctgct cccgccccga aaaagggctc caagaaggcg
      841 gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat
      901 tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag
      961 gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct
     1021 tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc
     1081 gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg tactaaggcc
     1141 atcaccaagt acaccagcgc taagtaagga tccaccggat ctagataact gatcataatc
     1201 agccatacca catt
//
