LOCUS       sequence-87062-e        1266 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-H3-23
FEATURES             Location/Qualifiers
     source          1..1266
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    430..482
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      490..499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             496..1230
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag ccgccaccat ggctcgtact aaacagacag ctcggaaatc caccggcggt
       61 aaagcgccac gcaagcagct ggctaccaag gctgctcgca agagcgcgcc ggctaccggc
      121 ggcgtgaaaa agcctcaccg ttaccgcccg ggcactgtgg ctctgcgcga gatccgccgc
      181 taccaaaagt cgaccgagtt gctgattcgg aagctgccgt tccagcgcct ggtgcgagaa
      241 atcgcccaag acttcaagac cgatcttcgc ttccagagct ctgcggtgat ggcgctgcag
      301 gaggcttgtg aggcctactt ggtagggctc tttgaggaca caaacctttg cgccatccat
      361 gctaagcgag tgactattat gcccaaagac atccagctcg ctcgccgcat tcgcggagaa
      421 agagcggatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      481 ccaccggtcg ccaccatggt gagcaagggc gaggagctga tcaaggagaa catgcacatg
      541 aagctgtaca tggaaggcac cgtgaacaac caccacttca agtgcaccac cgaaggggag
      601 ggcaagccct acgagggcac ccagacccag aggattaagg tggtggaggg aggccccctg
      661 ccgttcgcat tcgacatcct ggccacctgc tttatgtacg ggagcaagac cttcatcaac
      721 cacacccagg gcatccccga tttctttaag cagtccttcc ctgagggctt cacatgggag
      781 agagtcacca catacgaaga cgggggcgtg cttaccgtta cccaggacac cagcctccag
      841 gacggctgct tgatctacaa cgtcaagctc agaggggtga acttcccatc caacggccct
      901 gtgatgcaga agaaaacact cggctgggag gccaccaccg agaccctgta ccccgctgac
      961 ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg tgggcggggg ccacctgcac
     1021 tgcaacctga agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
     1081 gtctactttg tggaccgcag actggaaaga atcaaggagg ccgacaatga gacctacgtc
     1141 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa
     1201 cttaatggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca
     1261 taccac
//
