LOCUS       sequence-87063-e        2122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1350..1359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1356..2090
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag caccatgagc ttcaccactc gctccacctt ctccaccaac taccggtccc
       61 tgggctctgt ccaggcgccc agctacggcg cccggccggt cagcagcgcg gccagcgtct
      121 atgcaggcgc tgggggctct ggttcccgga tctccgtgtc ccgctccacc agcttcaggg
      181 gcggcatggg gtccgggggc ctggccaccg ggatagccgg gggtctggca ggaatgggag
      241 gcatccagaa cgagaaggag accatgcaaa gcctgaacga ccgcctggcc tcttacctgg
      301 acagagtgag gagcctggag accgagaacc ggaggctgga gagcaaaatc cgggagcact
      361 tggagaagaa gggaccccag gtcagagact ggagccatta cttcaagatc atcgaggacc
      421 tgagggctca gatcttcgca aatactgtgg acaatgcccg catcgttctg cagattgaca
      481 atgcccgtct tgctgctgat gactttagag tcaagtatga gacagagctg gccatgcgcc
      541 agtctgtgga gaacgacatc catgggctcc gcaaggtcat tgatgacacc aatatcacac
      601 gactgcagct ggagacagag atcgaggctc tcaaggagga gctgctcttc atgaagaaga
      661 accacgaaga ggaagtaaaa ggcctacaag cccagattgc cagctctggg ttgaccgtgg
      721 aggtagatgc ccccaaatct caggacctcg ccaagatcat ggcagacatc cgggcccaat
      781 atgacgagct ggctcggaag aaccgagagg agctagacaa gtactggtct cagcagattg
      841 aggagagcac cacagtgctc accacacagt ctgctgaggt tggagctgct gagacgacgc
      901 tcacagagct gagacgtaca gtccagtcct tggagatcga cctggactcc atgagaaatc
      961 tgaaggccag cttggagaac agcctgaggg aggtggaggc ccgctacgcc ctacagatgg
     1021 agcagctcaa cgggatcctg ctgcaccttg agtcagagct ggcacagacc cgggcagagg
     1081 gacagcgcca ggcccaggag tatgaggccc tgctgaacat caaggtcaag ctggaggctg
     1141 agatcgccac ctaccgccgc ctgctggaag atggcgagga ctttaatctt ggtgatgcct
     1201 tggacagcag caactccatg caaaccatcc aaaagaccac cacccgccgg atagtggatg
     1261 gcaaagtggt gtctgagacc aatgacacca aagttctgag gcatcgaatt ctgcagtcga
     1321 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctga
     1381 tcaaggagaa catgcacatg aagctgtaca tggaaggcac cgtgaacaac caccacttca
     1441 agtgcaccac cgaaggggag ggcaagccct acgagggcac ccagacccag aggattaagg
     1501 tggtggaggg aggccccctg ccgttcgcat tcgacatcct ggccacctgc tttatgtacg
     1561 ggagcaagac cttcatcaac cacacccagg gcatccccga tttctttaag cagtccttcc
     1621 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg cttaccgtta
     1681 cccaggacac cagcctccag gacggctgct tgatctacaa cgtcaagctc agaggggtga
     1741 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gccaccaccg
     1801 agaccctgta ccccgctgac ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg
     1861 tgggcggggg ccacctgcac tgcaacctga agaccacata cagatccaag aaacccgcta
     1921 agaacctcaa gatgcccggc gtctactttg tggaccgcag actggaaaga atcaaggagg
     1981 ccgacaatga gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
     2041 ctagcaaact ggggcacaaa cttaatggca tggacgagct gtacaagtaa gcggccgcga
     2101 ctctagatca taatcagcca ta
//
