LOCUS       sequence-87064-e        2598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..763
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc aagggcgagg agctgatcaa
       61 ggagaacatg cacatgaagc tgtacatgga aggcaccgtg aacaaccacc acttcaagtg
      121 caccaccgaa ggggagggca agccctacga gggcacccag acccagagga ttaaggtggt
      181 ggagggaggc cccctgccgt tcgcattcga catcctggcc acctgcttta tgtacgggag
      241 caagaccttc atcaaccaca cccagggcat ccccgatttc tttaagcagt ccttccctga
      301 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctta ccgttaccca
      361 ggacaccagc ctccaggacg gctgcttgat ctacaacgtc aagctcagag gggtgaactt
      421 cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca ccaccgagac
      481 cctgtacccc gctgacggcg gcctggaagg cagatgcgac atggccctga agctcgtggg
      541 cgggggccac ctgcactgca acctgaagac cacatacaga tccaagaaac ccgctaagaa
      601 cctcaagatg cccggcgtct actttgtgga ccgcagactg gaaagaatca aggaggccga
      661 caatgagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag
      721 caaactgggg cacaaactta atggcatgga cgagctgtac aagtccggac tcagatctcg
      781 agctcaagct tcgatggcga ctgcgacccc cgtgccgccg cggatgggca gccgcgctgg
      841 cggccccacc acgccgctga gccccacgcg cctgtcgcgg ctccaggaga aggaggagct
      901 gcgcgagctc aatgaccggc tggcggtgta catcgacaag gtgcgcagcc tggagacgga
      961 gaacagcgcg ctgcagctgc aggtgacgga gcgcgaggag gtgcgcggcc gtgagctcac
     1021 cggcctcaag gcgctctacg agaccgagct ggccgacgcg cgacgcgcgc tcgacgacac
     1081 ggcccgcgag cgcgccaagc tgcagatcga gctgggcaag tgcaaggcgg aacacgacca
     1141 gctgctcctc aactatgcta agaaggaatc tgatcttaat ggcgcccaga tcaagcttcg
     1201 agaatatgaa gcagcactca attcgaaaga tgcagctctt gctactgcac ttggtgacaa
     1261 aaaaagttta gagggagatt tggaggatct gaaggatcag attgcccagt tggaagcctc
     1321 cttagctgca gccaaaaaac agttagcaga tgaaacttta cttaaagtag atttggagaa
     1381 tcgttgtcag agccttactg aggacttgga gtttcgcaaa agcatgtatg aagaggagat
     1441 taacgagacc agaaggaagc atgaaacgcg cttggtagag gtggattctg ggcgtcaaat
     1501 tgagtatgag tacaagctgg cgcaagccct tcatgagatg agagagcaac atgatgccca
     1561 agtgaggctg tataaggagg agctggagca gacttaccat gccaaacttg agaatgccag
     1621 actgtcatca gagatgaata cttctactgt caacagtgcc agggaagaac tgatggaaag
     1681 ccgcatgaga attgagagcc tttcatccca gctttctaat ctacagaaag agtctagagc
     1741 atgtttggaa aggattcaag aattagagga cttgcttgct aaagaaaaag acaactctcg
     1801 tcgcatgctg acagacaaag agagagagat ggcggaaata agggatcaaa tgcagcaaca
     1861 gctgaatgac tatgaacagc ttcttgatgt aaagttagcc ctggacatgg aaatcagtgc
     1921 ttacaggaaa ctcttagaag gcgaagaaga gaggttgaag ctgtctccaa gcccttcttc
     1981 ccgtgtgaca gtatcccgag catcctcaag tcgtagtgta cgtacaacta gaggaaagcg
     2041 gaagagggtt gatgtggaag aatcagaggc gagtagtagt gttagcatct ctcattccgc
     2101 ctcagccact ggaaatgttt gcatcgaaga aattgatgtt gatgggaaat ttatccgctt
     2161 gaagaacact tctgaacagg atcaaccaat gggaggctgg gagatgatca gaaaaattgg
     2221 agacacatca gtcagttata aatatacctc aagatatgtg ctgaaggcag gccagactgt
     2281 tacaatttgg gctgcaaacg ctggtgtcac agccagcccc ccaactgacc tcatctggaa
     2341 gaaccagaac tcgtggggca ctggcgaaga tgtgaaggtt atattgaaaa attctcaggg
     2401 agaggaggtt gctcaaagaa gtacagtctt taaaacaacc atacctgaag aagaggagga
     2461 ggaggaagaa gcagctggag tggttgttga ggaagaactt ttccaccagc agggaacccc
     2521 aagagcatcc aatagaagct gtgcaattat gtagggatcc accggatcta gataactgat
     2581 cataatcagc cataccac
//
