LOCUS       sequence-87065-e        2092 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2092
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(41..103,104..1261)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1316..1325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1322..2056
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagcgccacc atggcggccc cgggcgcccg
       61 gcgtccgctg ctcctgttgc tgctggcagg ccttgcacac agcgccccag cactgttcga
      121 ggtgaaagac aacaacggca cagcgtgtat aatggccagc ttctctgcct cctttctgac
      181 cacctatgag gctggacatg tttctaaggt ctcgaatatg accctgccag cctctgcaga
      241 agtcctgaag aatagcagct cttgtggtga aaagaatgct tctgagccca ccctcgcaat
      301 cacctttgga gaaggatatt tactgaaact caccttcaca aaaaacacaa cacgttacag
      361 tgtccagcac atgtatttca catataacct gtcagacaca caattctttc ccaatgccag
      421 ctccaaaggg cccgacactg tggattccac aactgacatc aaggcagaca tcaacaaaac
      481 ataccgatgt gtcagcgaca tcagggtcta catgaagaat gtgaccattg tgctctggga
      541 cgctactatc caggcctacc tgccgagtag caacttcagc aaggaagaga cacgctgccc
      601 acaggatcaa ccttccccaa ctactgggcc acccagcccc tcaccaccac ttgtgcccac
      661 aaaccccagt gtgtccaagt acaatgtgac tggtgacaat ggaacctgcc tgctggcctc
      721 tatggcactg caactcaaca tcacctacat gaagaaggac aacacgactg tgaccagagc
      781 attcaacatc aacccaagtg acaaatatag tgggacttgc ggtgcccagt tggtgaccct
      841 gaaggtgggg aacaagagca gagtcctgga gctgcagttt gggatgaatg ccacttctag
      901 cctgtttttc ctgcaaggag ttcagttgaa catgactctt cctgatgcca tagagcccac
      961 gttcagcacc tccaactatt ccctgaaagc tcttcaggcc agtgtcggca actcatacaa
     1021 gtgcaacagt gaggagcaca tctttgtcag caaggcgctc gccctcaatg tcttcagcgt
     1081 gcaagtccag gctttcaggg tagaaagtga caggtttggg tctgtggaag agtgtgtaca
     1141 ggacggtaac aacatgctga tccccattgc tgtgggcggg gccctggcag ggctggtcct
     1201 catcgtcctc atcgcctacc tcatcggcag gaagaggagt cacgcgggct atcagaccat
     1261 ctcggaattc ggctccaccg gctccaccgg ctccaccggc gcggatccac cggtcgccac
     1321 catggtgagc aagggcgagg agctgatcaa ggagaacatg cacatgaagc tgtacatgga
     1381 aggcaccgtg aacaaccacc acttcaagtg caccaccgaa ggggagggca agccctacga
     1441 gggcacccag acccagagga ttaaggtggt ggagggaggc cccctgccgt tcgcattcga
     1501 catcctggcc acctgcttta tgtacgggag caagaccttc atcaaccaca cccagggcat
     1561 ccccgatttc tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     1621 cgaagacggg ggcgtgctta ccgttaccca ggacaccagc ctccaggacg gctgcttgat
     1681 ctacaacgtc aagctcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     1741 aacactcggc tgggaggcca ccaccgagac cctgtacccc gctgacggcg gcctggaagg
     1801 cagatgcgac atggccctga agctcgtggg cgggggccac ctgcactgca acctgaagac
     1861 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actttgtgga
     1921 ccgcagactg gaaagaatca aggaggccga caatgagacc tacgtcgagc agcacgaggt
     1981 ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta atggcatgga
     2041 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc ac
//
