LOCUS       sequence-87066-e        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      375..384
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             381..1115
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 ccgctagcgc caccatgaag ttaagtcgcc agttcaccgt gtttggcagc gcgatcttct
       61 gcgtcgtaat cttctcactc tacctgatgc tggacagggg tcacttggac taccctcggg
      121 gcccgcgcca ggagggctcc tttccgcagg gccagctttc aatattgcaa gaaaagattg
      181 accatttgga gcgtttgctc gctgagaaca acgagatcat ctcaaatatc agagactcag
      241 tcatcaacct gagcgagtct gtggaggacg gcccgcgggg gtcaccaggc aacgccagcc
      301 aaggctccat ccacctccac tcgccacagt tggccctgca ggctgacccc agctccggat
      361 cggatccacc ggtcgccacc atggtgagca agggcgagga gctgatcaag gagaacatgc
      421 acatgaagct gtacatggaa ggcaccgtga acaaccacca cttcaagtgc accaccgaag
      481 gggagggcaa gccctacgag ggcacccaga cccagaggat taaggtggtg gagggaggcc
      541 ccctgccgtt cgcattcgac atcctggcca cctgctttat gtacgggagc aagaccttca
      601 tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag ggcttcacat
      661 gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag gacaccagcc
      721 tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc ccatccaacg
      781 gccctgtgat gcagaagaaa acactcggct gggaggccac caccgagacc ctgtaccccg
      841 ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc gggggccacc
      901 tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
      961 ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa ggaggccgac aatgagacct
     1021 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc
     1081 acaaacttaa tggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
     1141 agccatacca c
//
