LOCUS       sequence-87067-e        3014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules/Kinesin, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-MCAK-C-7
FEATURES             Location/Qualifiers
     source          1..3014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..776
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gcatgctagt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg
       61 aggagctgat caaggagaac atgcacatga agctgtacat ggaaggcacc gtgaacaacc
      121 accacttcaa gtgcaccacc gaaggggagg gcaagcccta cgagggcacc cagacccaga
      181 ggattaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg gccacctgct
      241 ttatgtacgg gagcaagacc ttcatcaacc acacccaggg catccccgat ttctttaagc
      301 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
      361 ttaccgttac ccaggacacc agcctccagg acggctgctt gatctacaac gtcaagctca
      421 gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
      481 ccaccaccga gaccctgtac cccgctgacg gcggcctgga aggcagatgc gacatggccc
      541 tgaagctcgt gggcgggggc cacctgcact gcaacctgaa gaccacatac agatccaaga
      601 aacccgctaa gaacctcaag atgcccggcg tctactttgt ggaccgcaga ctggaaagaa
      661 tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg gccagatact
      721 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtccg
      781 gagccgcttc cgcctccatg gccatggact cgtcgcttca ggcccgcctg tttcccggtc
      841 tcgctatcaa gatccaacgc agtaatggtt taattcacag tgccaatgta aggactgtga
      901 acttggagaa atcctgtgtt tcagtggaat gggcagaagg aggtgccaca aagggcaaag
      961 agattgattt tgatgatgtg gctgcaataa acccagaact cttacagctt cttcccttac
     1021 atccgaagga caatctgccc ttgcaggaaa atgtaacaat ccagaaacaa aaacggagat
     1081 ccgtcaactc caaaattcct gctccaaaag aaagtcttcg aagccgctcc actcgcatgt
     1141 ccactgtctc agagcttcgc atcacggctc aggagaatga catggaggtg gagctgcctg
     1201 cagctgcaaa ctcccgcaag cagttttcag ttcctcctgc ccccactagg ccttcctgcc
     1261 ctgcagtggc tgaaatacca ttgaggatgg tcagcgagga gatggaagag caagtccatt
     1321 ccatccgagg cagctcttct gcaaaccctg tgaactcagt tcggaggaaa tcatgtcttg
     1381 tgaaggaagt ggaaaaaatg aagaacaagc gagaagagaa gaaggcccag aactctgaaa
     1441 tgagaatgaa gagagctcag gagtatgaca gtagttttcc aaactgggaa tttgcccgaa
     1501 tgattaaaga atttcgggct actttggaat gtcatccact tactatgact gatcctatcg
     1561 aagagcacag aatatgtgtc tgtgttagga aacgcccact gaataagcaa gaattggcca
     1621 agaaagaaat tgatgtgatt tccattccta gcaagtgtct cctcttggta catgaaccca
     1681 agttgaaagt ggacttaaca aagtatctgg agaaccaagc attctgcttt gactttgcat
     1741 ttgatgaaac agcttcgaat gaagttgtct acaggttcac agcaaggcca ctggtacaga
     1801 caatctttga aggtggaaaa gcaacttgtt ttgcatatgg ccagacagga agtggcaaga
     1861 cacatactat gggcggagac ctctctggga aagcccagaa tgcatccaaa gggatctatg
     1921 ccatggcctc ccgggacgtc ttcctcctga agaatcaacc ctgctaccgg aagttgggcc
     1981 tggaagtcta tgtgacattc ttcgagatct acaatgggaa gctgtttgac ctgctcaaca
     2041 agaaggccaa gctgcgcgtg ctggaggacg gcaagcaaca ggtgcaagtg gtggggctgc
     2101 aggagcatct ggttaactct gctgatgatg tcatcaagat gatcgacatg ggcagcgcct
     2161 gcagaacctc tgggcagaca tttgccaact ccaattcctc ccgctcccac gcgtgcttcc
     2221 aaattattct tcgagctaaa gggagaatgc atggcaagtt ctctttggta gatctggcag
     2281 ggaatgagcg aggcgcggac acttccagtg ctgaccggca gacccgcatg gagggcgcag
     2341 aaatcaacaa gagtctctta gccctgaagg agtgcatcag ggccctggga cagaacaagg
     2401 ctcacacccc gttccgtgag agcaagctga cacaggtgct gagggactcc ttcattgggg
     2461 agaactctag gacttgcatg attgccacga tctcaccagg cataagctcc tgtgaatata
     2521 ctttaaacac cctgagatat gcagacaggg tcaaggagct gagcccccac agtgggccca
     2581 gtggagagca gttgattcaa atggaaacag aagagatgga agcctgctct aacggggcgc
     2641 tgattccagg caatttatcc aaggaagagg aggaactgtc ttcccagatg tccagcttta
     2701 acgaagccat gactcagatc agggagctgg aggagaaggc tatggaagag ctcaaggaga
     2761 tcatacagca aggaccagac tggcttgagc tctctgagat gaccgagcag ccagactatg
     2821 acctggagac ctttgtgaac aaagcggaat ctgctctggc ccagcaagcc aagcatttct
     2881 cagccctgcg agatgtcatc aaggccttgc gcctggccat gcagctggaa gagcaggcta
     2941 gcagacaaat aagcagcaag aaacggcccc agtgactcga gctcaagctt cgaattctgc
     3001 agtcgacggt accg
//
