LOCUS       sequence-87068-e        2339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..759
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gatcaaggag
       61 aacatgcaca tgaagctgta catggaaggc accgtgaaca accaccactt caagtgcacc
      121 accgaagggg agggcaagcc ctacgagggc acccagaccc agaggattaa ggtggtggag
      181 ggaggccccc tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag
      241 accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac
      361 accagcctcc aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaccac cgagaccctg
      481 taccccgctg acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg
      541 ggccacctgc actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc
      601 aagatgcccg gcgtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat
      661 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca aacttaatgg catggacgag ctgtacaagt ccggaagtgc tgggagcgca
      781 agcggaagcg gaggctccgg ttttaactac gaacgtccaa aacacttcat ccagtcccaa
      841 aacccatgtg gctccagatt gcagcctcct ggaccagaaa cctccagctt ctctagccag
      901 accaaacagt cttccattat catccagccc cgccagtgta cagagcaaag attttctgcc
      961 tcctcaacac tgagctctca catcaccatg tcctcctctg ctttccctgc ttctccccag
     1021 cagcatgctg gctccaaccc aggccaaagg gttacaacca cctataacca gtccccagcc
     1081 agcttcctca gctccatatt accatcacag cctgattaca atagcagtaa aatcccttcc
     1141 gctatggatt ccaactatca acagtcctca gctggccaac ctataaatgc aaagccatcc
     1201 caaactgcaa atgctaagcc cataccaaga actcctgatc atgaaataca aggatcaaaa
     1261 gaagctttga ttcaagattt ggaaagaaag ctgaaatgca aggacaccct tcttcataat
     1321 ggaaatcaac gtctaacata tgaagagaag atggctcgca gattgctagg accacagaat
     1381 gcagctgctg tgtttcaagc tcaggatgac agtggtgcac aagactcgca gcaacacaac
     1441 tcagaacatg cgcgactgca agttcctaca tcacaagtaa gaagtagatc aacctcaagg
     1501 ggagatgtga atgatcagga tgcaatccag gagaaatttt acccaccacg tttcattcaa
     1561 gtgccagaga acatgtcgat tgatgaagga agattctgca gaatggactt caaagtgagt
     1621 ggactgccag ctcctgatgt gtcatggtat ctaaatggaa gaacagttca atcagatgat
     1681 ttgcacaaaa tgatagtgtc tgagaagggt cttcattcac tcatctttga agtagtcaga
     1741 gcttcagatg caggggctta tgcatgtgtt gccaagaata gagcaggaga agccaccttc
     1801 actgtgcagc tggatgtcct tgcaaaagaa cataaaagag caccaatgtt tatctacaaa
     1861 ccacagagca aaaaagtttt agagggagat tcagtgaaac tagaatgcca gatctcggct
     1921 atacctccac caaagctttt ctggaaaaga aataatgaaa tggtacaatt caacactgac
     1981 cgaataagct tatatcaaga taacactgga agagttactt tactgataaa agatgtaaac
     2041 aagaaagatg ctgggtggta tactgtgtca gcagttaatg aagctggagt gactacatgt
     2101 aacacaagat tagacgttac ggcacgtcca aaccaaactc ttccagctcc taagcagtta
     2161 cgggttcgac caacattcag caaatattta gcacttaatg ggaaaggttt gaatgtaaaa
     2221 caagctttta acccagaagg agaatttcag cgtttggcag ctcaatctgg actctatgaa
     2281 agtgaagaac tttaaggatc caccggatct agataactga tcataatcag ccataccac
//
