LOCUS       sequence-87069-e        2225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-NUP50-N-10
FEATURES             Location/Qualifiers
     source          1..2225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1459..1468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1465..2199
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 aacggtcaga ttccgcttag cgatgccacc atggccaaaa gaaatgccga gaaggaactg
       61 acagatagga attgggatca agaagatgaa gctgaagagg tgggaacatt ctccatggcc
      121 agtgaggaag tcttgaagaa tagagccata aagaaagcaa agcgcagaaa tgttggattt
      181 gaatctgaca ctggaggagc ctttaaaggt tttaaaggtt tggtggtacc ttctggagga
      241 ggacgctttt ctggatttgg tagtggcgct ggagggaagc ctctggaagg actgtcgaat
      301 ggaaacaaca taaccagtgc ccctcccttc gccagtgcaa aggcagcggc agatcccaag
      361 gtagcctttg gttctcttgc tgcaaatggc cctaccacct tggttgataa agtttcaaat
      421 cccaaaacta atggggacag tcagcagccc tcctcctctg gccttgcttc cagtaaagct
      481 tgtgtcggaa atgcctatca caagcagttg gccgccttga actgctccgt gcgggattgg
      541 atagtgaagc acgtgaatac aaaccccctc tgtgatctga cacctatctt taaagactat
      601 gagaaatatt tagcaaacat tgaacagcaa cacgggaaca gtggcaggaa ttctgaaagt
      661 gaatctaaca aagtggcagc tgaaacacag tctccttccc tttttggctc aacaaaatta
      721 cagcaagagt caacgttttt gtttcatggc aacaaaactg aagatacacc tgacaagaag
      781 atggaggtgg catctgaaaa gaaaacggac ccatcatcac taggagcgac aagtgcctca
      841 tttaatttcg gcaagaaagt tgatagctct gttttgggct cattaagctc tgtccccctg
      901 actggatttt ctttctcccc tggaaactcc agtttatttg gcaaagatac tacccagagt
      961 aaaccagtct cttcaccatt tcccactaaa ccattggagg gccaagcaga aggtgacagt
     1021 ggtgaatgca aaggtggaga tgaagaagag aatgatgagc cacccaaagt agtagttacc
     1081 gaagtaaaag aagaagacgc tttttactcc aaaaagtgta aactgtttta caagaaagac
     1141 aatgagttta aagagaaagg cataggtact ctgcatttaa aacctacagc aaatcagaag
     1201 acacagcttt tggtgcgggc agacaccaat ttaggcaaca tattgctgaa cgttctgatt
     1261 ccacccaata tgccatgtac gcgaacaggg aagaataacg ttcttatcgt ctgtgttcca
     1321 aatccaccaa ttgacgagaa gaatgccacc atgccagtca ccatgttgat tcgggtaaaa
     1381 accagcgagg atgcagacga gttgcacaaa attttactgg agaaaaagga tgccagctcc
     1441 ggaggggatc caccggtcgc caccatggtg agcaagggcg aggagctgat caaggagaac
     1501 atgcacatga agctgtacat ggaaggcacc gtgaacaacc accacttcaa gtgcaccacc
     1561 gaaggggagg gcaagcccta cgagggcacc cagacccaga ggattaaggt ggtggaggga
     1621 ggccccctgc cgttcgcatt cgacatcctg gccacctgct ttatgtacgg gagcaagacc
     1681 ttcatcaacc acacccaggg catccccgat ttctttaagc agtccttccc tgagggcttc
     1741 acatgggaga gagtcaccac atacgaagac gggggcgtgc ttaccgttac ccaggacacc
     1801 agcctccagg acggctgctt gatctacaac gtcaagctca gaggggtgaa cttcccatcc
     1861 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaccaccga gaccctgtac
     1921 cccgctgacg gcggcctgga aggcagatgc gacatggccc tgaagctcgt gggcgggggc
     1981 cacctgcact gcaacctgaa gaccacatac agatccaaga aacccgctaa gaacctcaag
     2041 atgcccggcg tctactttgt ggaccgcaga ctggaaagaa tcaaggaggc cgacaatgag
     2101 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     2161 gggcacaaac ttaatggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     2221 aatca
//
