LOCUS       sequence-87071-e        1988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondrial Matrix, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1215..1224
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1221..1955
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag caccgccacc atgaggaaga tgctcgccgc cgtctcccgc gtgctgtctg
       61 gcgcttctca gaagccggca agcagagtgc tggtagcatc ccgtaatttt gcaaatgatg
      121 ctacatttga aattaagaaa tgtgaccttc accggctgga agaaggccct cctgtcacaa
      181 cagtgctcac cagggaggat gggctcaaat actacaggat gatgcagact gtacgccgaa
      241 tggagttgaa agcagatcag ctgtataaac agaaaattat tcgtggtttc tgtcacttgt
      301 gtgatggtca ggaagcttgc tgtgtgggcc tggaggccgg catcaacccc acagaccatc
      361 tcatcacagc ctaccgggct cacggcttta ctttcacccg gggcctttcc gtccgagaaa
      421 ttctcgcaga gcttacagga cgaaaaggag gttgtgctaa agggaaagga ggatcgatgc
      481 acatgtatgc caagaacttc tacgggggca atggcatcgt gggagcgcag gtgcccctgg
      541 gcgctgggat tgctctagcc tgtaagtata atggaaaaga tgaggtctgc ctgactttat
      601 atggcgatgg tgctgctaac cagggccaga tattcgaagc ttacaacatg gcagctttgt
      661 ggaaattacc ttgtattttc atctgtgaga ataatcgcta tggaatggga acgtctgttg
      721 agagagcggc agccagcact gattactaca agagaggcga tttcattcct gggctgagag
      781 tggatggaat ggatatcctg tgcgtccgag aggcaacaag gtttgctgct gcctattgta
      841 gatctgggaa ggggcccatc ctgatggagc tgcagactta ccgttaccac ggacacagta
      901 tgagtgaccc tggagtcagt taccgtacac gagaagaaat tcaggaagta agaagtaaga
      961 gtgaccctat tatgcttctc aaggacagga tggtgaacag caatcttgcc agtgtggaag
     1021 aactaaagga aattgatgtg gaagtgagga aggagattga ggatgctgcc cagtttgcca
     1081 cggccgatcc tgagccacct ttggaagagc tgggctacca catctactcc agcgacccac
     1141 cttttgaagt tcgtggtgcc aatcagtgga tcaagtttaa gtcagtcagt agctccggag
     1201 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgatcaag gagaacatgc
     1261 acatgaagct gtacatggaa ggcaccgtga acaaccacca cttcaagtgc accaccgaag
     1321 gggagggcaa gccctacgag ggcacccaga cccagaggat taaggtggtg gagggaggcc
     1381 ccctgccgtt cgcattcgac atcctggcca cctgctttat gtacgggagc aagaccttca
     1441 tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag ggcttcacat
     1501 gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag gacaccagcc
     1561 tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc ccatccaacg
     1621 gccctgtgat gcagaagaaa acactcggct gggaggccac caccgagacc ctgtaccccg
     1681 ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc gggggccacc
     1741 tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc
     1801 ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa ggaggccgac aatgagacct
     1861 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc
     1921 acaaacttaa tggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc
     1981 agccatac
//
