LOCUS       sequence-87072-e        1417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      645..654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             651..1385
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctg ccaccatggc gccggccgcg tccaggctgc
       61 gggccgaagc cgggctcggg gcgctgccgc ggcgggcgct cgcccagtac ttgctcttcc
      121 tgcggctcta cccggtgctc accaaggcgg ccaccagtgg cattttgtca gcacttggga
      181 acttcctggc ccagatgatt gagaagaagc ggaaaaaaga aaactctaga agtctggatg
      241 tcggtgggcc tctgagatat gccgtttacg ggttcttctt cacagggccg ctgagtcact
      301 tcttctactt cttcatggaa cattggatcc ctcctgaggt ccccctggca gggctcagga
      361 ggcttctcct ggaccgcctc gtctttgcac cggccttcct catgttgttc ttcctcatca
      421 tgaactttct ggaggggaaa gacgcctcag ccttcgccgc caagatgagg gggggcttct
      481 ggccggcgct gaggatgaac tggcgggtgt ggacgccact acagttcatc aacatcaact
      541 acgtccctct gaagttccgg gtgctcttcg ccaacctggc agctctgttc tggtatgcct
      601 acctggcctc cttggggaag agcggctccg gtagcggacc ggtcgccacc atggtgagca
      661 agggcgagga gctgatcaag gagaacatgc acatgaagct gtacatggaa ggcaccgtga
      721 acaaccacca cttcaagtgc accaccgaag gggagggcaa gccctacgag ggcacccaga
      781 cccagaggat taaggtggtg gagggaggcc ccctgccgtt cgcattcgac atcctggcca
      841 cctgctttat gtacgggagc aagaccttca tcaaccacac ccagggcatc cccgatttct
      901 ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
      961 gcgtgcttac cgttacccag gacaccagcc tccaggacgg ctgcttgatc tacaacgtca
     1021 agctcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct
     1081 gggaggccac caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca
     1141 tggccctgaa gctcgtgggc gggggccacc tgcactgcaa cctgaagacc acatacagat
     1201 ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctttgtggac cgcagactgg
     1261 aaagaatcaa ggaggccgac aatgagacct acgtcgagca gcacgaggtg gctgtggcca
     1321 gatactgcga cctccctagc aaactggggc acaaacttaa tggcatggac gagctgtaca
     1381 agtaagcggc cgcgactcta gatcataatc agccata
//
