LOCUS       sequence-87073-e        1471 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1471
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..759
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gatcaaggag
       61 aacatgcaca tgaagctgta catggaaggc accgtgaaca accaccactt caagtgcacc
      121 accgaagggg agggcaagcc ctacgagggc acccagaccc agaggattaa ggtggtggag
      181 ggaggccccc tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag
      241 accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac
      361 accagcctcc aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaccac cgagaccctg
      481 taccccgctg acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg
      541 ggccacctgc actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc
      601 aagatgcccg gcgtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat
      661 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca aacttaatgg catggacgag ctgtacaagt ccggactcag atctcgagcc
      781 atgtcgcaga cggccatgtc cgaaacctac gattttttgt ttaagttctt ggttattgga
      841 aatgcaggaa ctggcaaatc ttgcttactt catcagttta ttgaaaaaaa attcaaagat
      901 gactcaaatc atacaatagg agtggaattt ggttcaaaga taataaatgt tggtggtaaa
      961 tatgtaaagt tacaaatatg ggatacagca ggacaagaac gattcaggtc cgtgacgaga
     1021 agttattacc gaggcgcggc cggggctctc ctcgtctatg atatcaccag ccgagaaacc
     1081 tacaatgcgc ttactaattg gttaacagat gcccgaatgc tggcgagcca gaacattgtg
     1141 atcatccttt gtggaaacaa gaaggacctg gatgcagatc gtgaagttac cttcttagaa
     1201 gcctccagat ttgctcaaga aaatgagctg atgtttttgg aaacaagtgc gctcacaggg
     1261 gagaatgtag aagaggcttt tgtacagtgt gcaagaaaaa tacttaacaa aatcgaatca
     1321 ggtgagctgg acccagaaag aatgggctca ggtattcagt acggagatgc tgccttgaga
     1381 cagctgaggt caccgcggcg cgcacaggcc ccgaacgctc aggagtgtgg ttgttaggga
     1441 tccaccggat ctagataact gatcataatc a
//
