LOCUS       sequence-87074-e        6065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Tensin1-N-18
FEATURES             Location/Qualifiers
     source          1..6065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5293..5302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5299..6033
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gaggccacca tgagtgtgag ccggaccatg
       61 gaggacagct gtgagctgga cctggtgtac gtcacagaga ggatcatcgc tgtctccttc
      121 cccagcacag ccaatgagga gaactttcgg agcaacctcc gtgaggtggc gcagatgctc
      181 aagtccaaac atggaggcaa ctacctgctg ttcaacctct ctgagcggag acctgacatc
      241 acgaagctcc atgccaaggt actggaattt ggctggcccg acctccacac cccagccctg
      301 gagaagatat gcagcatctg taaggccatg gacacatggc tcaatgcaga ccctcacaat
      361 gtcgttgttc tacacaacaa gggaaaccga ggcaggatag gagttgtcat cgcggcttac
      421 atgcactaca gcaacatttc tgccagtgcg gaccaggctc tggatcggtt tgcaatgaag
      481 cggttctatg aggataagat tgtgcccatt ggccagccat cccaaagaag gtacgtgcat
      541 tacttcagtg gcctgctctc cggctccatc aaaatgaaca acaagccctt gtttctgcac
      601 cacgtgatca tgcacggcat ccccaacttt gagtctaaag gaggatgtcg gccatttctc
      661 cgcatctacc aggccatgca acctgtgtac acatctggca tctacaacat cccaggagac
      721 agccagacta gcgtctgcat caccatcgag ccaggactgc tcttgaaggg agacatcttg
      781 ctgaagtgct accacaagaa gttccgaagc ccagcccgag acgtcatctt ccgtgtgcag
      841 ttccacacct gtgccatcca tgacctgggg gttgtctttg ggaaggagga ccttgatgat
      901 gctttcaaag atgatcgatt tccagagtat ggcaaagtgg agtttgtatt ttcttatggg
      961 ccagagaaaa ttcaaggcat ggagcacctg gagaacgggc cgagcgtgtc tgtggactat
     1021 aacacctccg accccctcat ccgctgggac tcctacgaca acttcagtgg gcatcgagat
     1081 gacggcatgg aggaggtggt gggacacacg caggggccac tagatgggag cctgtatgct
     1141 aaggtgaaga agaaagactc cctgcacggc agcaccgggg ctgttaatgc cacacgtcct
     1201 acactgtcgg ccacccccaa ccacgtggaa cacacgcttt ctgtgagcag cgactcgggc
     1261 aactccacag cctccaccaa gaccgacaag accgacgagc ctgtccccgg ggcctccagt
     1321 gccactgctg ccttgagtcc ccaggagaag cgggagctgg accgcctgct gagtggcttt
     1381 ggcttagagc gagagaagca aggcgccatg taccacaccc agcacctcag gtcccgccca
     1441 gcagggggct cggctgtgcc ctcctctgga cgccacgttg tcccagccca ggttcatgtc
     1501 aatggtgggg cgttagcatc tgagcgggag acagacatcc tggacgatga attgccaaac
     1561 caggatggtc acagtgcggg cagcatgggc acactctctt ctctggacgg ggtcaccaac
     1621 accagtgagg ggggctaccc agaggccctg tccccactga ccaacggtct ggacaagtcc
     1681 taccccatgg agcctatggt caatggagga ggctacccct acgagtctgc cagccgggcg
     1741 gggcctgccc atgctggcca cacggccccc atgcggccct cctactctgc acaggagggt
     1801 ttagctggct accagaggga ggggccccac ccagcctggc cacagccagt gaccacctcc
     1861 cactatgccc atgaccccag cggtatgttc cgctctcaat ccttttcgga agctgaaccc
     1921 cagctgcccc cagctccggt ccgaggggga agcagccggg aggctgtgca aaggggactg
     1981 aactcgtggc agcagcagca gcagcagcag cagcagcctc gcccacctcc acgccagcag
     2041 gaaagagccc acttggagag tcttgtagcc agcaggccca gccctcagcc attggcagag
     2101 acccccatcc ccagtctccc tgagttcccg cgagcagcct cccagcagga gattgaacag
     2161 tccatcgaaa cactcaatat gctgatgctg gacctggagc cagcctccgc tgctgcccca
     2221 ctacacaagt cccagagtgt ccccggggcc tggccagggg cttctccact ctcctcccag
     2281 cccctctctg ggtcctcccg tcagtcccat ccactgaccc agtccagata tggctatatc
     2341 cccagtgggc attcgttggg aacccctgag ccagccccac gggcctctct ggagtctgtc
     2401 cctcctggca ggtcttactc accttatgac tatcagccat gtttggctgg gcctaaccag
     2461 gatttccatt caaagagccc agcctcttcc tccttgcctg ccttccttcc gaccacccac
     2521 agccctccag ggcctcagca acccccagcc tctctccctg gcctcactgc tcagcctctg
     2581 ctctcaccaa aggaagcgac ttcagacccc tcccggactc cagaggagga gccattgaat
     2641 ttagaagggc tggtggccca cagggtagca ggggtacagg ctcgggagaa gcagcctgca
     2701 gagcccccag cccctctgcg gaggcgggcg gccagtgatg gacagtatga gaaccagtct
     2761 ccagaagcca catcccctcg tagccctggg gttcgctccc ctgtccagtg tgtctccccg
     2821 gagctggctc ttaccatcgc tctcaatcct ggagggcggc ccaaagagcc ccatttgcac
     2881 agctacaagg aggccttcga ggagatggag ggaacctccc cgagcagccc accacccagt
     2941 ggggtgcggt cccccccggg tctggccaag acacccctgt ctgctctggg cctgaaacct
     3001 cacaacccag cggacatcct gttgcacccc acaggagtta ccagaagacg gattcagcca
     3061 gaggaagatg aggggaaggt ggtggtcagg ctgtcagaag agccccggag ctatgtggag
     3121 tctgtggcac ggacagcggt ggctggaccc cgagctcagg actctgagcc caagagcttt
     3181 agtgctccag ccacccaggc ctatggccat gagatacccc tgaggaacgg gaccctgggt
     3241 ggctcctttg tctcccccag ccccctctcc accagcagcc ccatcctcag tgctgacagc
     3301 acttcagtgg ggagtttccc gtcgggagag agcagtgacc agggtccccg gacgcccacc
     3361 cagcctctgt tggagtctgg cttccgctca ggcagcctgg gacagcccag cccgtctgcc
     3421 cagagaaact accagagctc ttctcctctc ccgactgtgg gcagtagcta cagcagcccc
     3481 gactactcac ttcagcattt cagctcctct ccagaaagcc aggctcgcgc tcagttcagt
     3541 gtggctggcg tccacacggt gcctgggagc cctcaggcgc gccacagaac agtgggcacc
     3601 aacactcccc ctagtcctgg cttcggctgg cgggccatca atcccagcat ggctgccccc
     3661 agcagtccca gtttgagcca tcaccagatg atgggtccac caggcactgg cttccatggt
     3721 agcactgtct ccagccccca gagcagtgca gcgaccaccc cggggagccc cagcctgtgt
     3781 cggcacccag caggggtcta ccaggtttct ggcctccaca acaaagtggc caccaccccg
     3841 gggagtccca gcctgggccg gcaccctggg gctcaccaag gcaacctggc ctccggtctt
     3901 catagcaatg caatagccag ccctggaagc cccagcctgg gccgtcacct cggagggtct
     3961 ggatctgtgg ttcccggcag cccctgcttg gaccggcatg tggcctatgg tggctattct
     4021 accccggagg atcggagacc cacactgtcc cggcagagca gtgcctctgg ctaccaggct
     4081 ccttccacgc cctccttccc tgtctcccct gcctactacc ctggcctgag cagccctgcc
     4141 acctccccgt caccagactc cgcagccttc cggcaaggga gcccaacacc agccttgcca
     4201 gagaagcgaa ggatgtcagt gggagaccgg gcaggcagcc tccccaacta tgccaccatc
     4261 aatgggaagg tgtcttcgcc tgtcgccagc ggcatgtcca gtcccagtgg gggcagcacc
     4321 gtctccttct cccacactct gcccgacttc tccaagtact ccatgccaga caacagcccg
     4381 gagacgcggg ctaaagtgaa gtttgtccag gacacttcta agtattggta caagcctgag
     4441 atttccaggg agcaggccat cgcgctcctc aaggaccagg agccgggggc cttcatcatc
     4501 cgcgacagtc actccttccg aggcgcgtac gggctggcca tgaaggtgtc ttcgccacct
     4561 ccaaccatca tgcagcagaa taaaaaagga gacatgaccc atgagctggt caggcatttt
     4621 ctgatagaga ctggccccag aggagtcaag ctcaagggct gccccaatga gccaaacttc
     4681 ggatcgctgt ctgccctggt ctaccagcac tccatcatcc cattggccct gccttgcaag
     4741 ctggtcattc caaaccgaga ccccacagat gaatcgaaag atagctccgg ccctgccaac
     4801 tcaactgcag acctgctgaa acaaggggca gcctgcaatg tgctcttcgt caactctgtg
     4861 gacatggagt cactcactgg gccacaggcc atctctaaag ccacatctga gacgttggct
     4921 gcagacccca cgccagctgc caccatcgtt cacttcaaag tctctgccca gggaatcact
     4981 ctgactgaca accagagaaa gctctttttc agacgccact accctctcaa cactgtcacc
     5041 ttctgtgacc tggacccaca ggaaagaaag tggatgaaaa cagagggtgg tgcccctgct
     5101 aagctcttcg gcttcgtggc ccggaagcag ggcagcacca cggacaacgc ctgccacctc
     5161 tttgctgagc ttgaccccaa ccagccggcc tctgccatcg tcaacttcgt ctccaaggtc
     5221 atgctgaatg ccggccaaaa gagaggtagc gcatccgggg gtgcgtcagg cggcagcgga
     5281 tctggaccgg tcgccaccat ggtgagcaag ggcgaggagc tgatcaagga gaacatgcac
     5341 atgaagctgt acatggaagg caccgtgaac aaccaccact tcaagtgcac caccgaaggg
     5401 gagggcaagc cctacgaggg cacccagacc cagaggatta aggtggtgga gggaggcccc
     5461 ctgccgttcg cattcgacat cctggccacc tgctttatgt acgggagcaa gaccttcatc
     5521 aaccacaccc agggcatccc cgatttcttt aagcagtcct tccctgaggg cttcacatgg
     5581 gagagagtca ccacatacga agacgggggc gtgcttaccg ttacccagga caccagcctc
     5641 caggacggct gcttgatcta caacgtcaag ctcagagggg tgaacttccc atccaacggc
     5701 cctgtgatgc agaagaaaac actcggctgg gaggccacca ccgagaccct gtaccccgct
     5761 gacggcggcc tggaaggcag atgcgacatg gccctgaagc tcgtgggcgg gggccacctg
     5821 cactgcaacc tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     5881 ggcgtctact ttgtggaccg cagactggaa agaatcaagg aggccgacaa tgagacctac
     5941 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
     6001 aaacttaatg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     6061 ccata
//
