LOCUS       sequence-87075-e        2211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..757
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctga tcaaggagaa
       61 catgcacatg aagctgtaca tggaaggcac cgtgaacaac caccacttca agtgcaccac
      121 cgaaggggag ggcaagccct acgagggcac ccagacccag aggattaagg tggtggaggg
      181 aggccccctg ccgttcgcat tcgacatcct ggccacctgc tttatgtacg ggagcaagac
      241 cttcatcaac cacacccagg gcatccccga tttctttaag cagtccttcc ctgagggctt
      301 cacatgggag agagtcacca catacgaaga cgggggcgtg cttaccgtta cccaggacac
      361 cagcctccag gacggctgct tgatctacaa cgtcaagctc agaggggtga acttcccatc
      421 caacggccct gtgatgcaga agaaaacact cggctgggag gccaccaccg agaccctgta
      481 ccccgctgac ggcggcctgg aaggcagatg cgacatggcc ctgaagctcg tgggcggggg
      541 ccacctgcac tgcaacctga agaccacata cagatccaag aaacccgcta agaacctcaa
      601 gatgcccggc gtctactttg tggaccgcag actggaaaga atcaaggagg ccgacaatga
      661 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
      721 ggggcacaaa cttaatggca tggacgagct gtacaagtcc ggactcagat ctggcagcgg
      781 tggaggcagc gcatccggcg gaagcggaag cgtgcgtgag tgcatctcca tccacgttgg
      841 ccaggctggt gtccagattg gcaatgcctg ctgggagctc tactgcctgg aacacggcat
      901 ccagcccgat ggccagatgc caagtgacaa gaccattggg ggaggagatg actccttcaa
      961 caccttcttc agtgagacgg gcgctggcaa gcacgtgccc cgggctgtgt ttgtagactt
     1021 ggaacccaca gtcattgatg aagttcgcac tggcacctac cgccagctct tccaccctga
     1081 gcagctcatc acaggcaagg aagatgctgc caataactat gcccgagggc actacaccat
     1141 tggcaaggag atcattgacc ttgtgttgga ccgaattcgc aagctggctg accagtgcac
     1201 cggtcttcag ggcttcttgg ttttccacag ctttggtggg ggaactggtt ctgggttcac
     1261 ctccctgctc atggaacgtc tctcagttga ttatggcaag aagtccaagc tggagttctc
     1321 catttaccca gcaccccagg tttccacagc tgtagttgag ccctacaact ccatcctcac
     1381 cacccacacc accctggagc actctgattg tgccttcatg gtagacaatg aggccatcta
     1441 tgacatctgt cgtagaaacc tcgatatcga gcgcccaacc tacactaacc ttaaccgcct
     1501 tattagccag attgtgtcct ccatcactgc ttccctgaga tttgatggag ccctgaatgt
     1561 tgacctgaca gaattccaga ccaacctggt gccctacccc cgcatccact tccctctggc
     1621 cacatatgcc cctgtcatct ctgctgagaa agcctaccat gaacagcttt ctgtagcaga
     1681 gatcaccaat gcttgctttg agccagccaa ccagatggtg aaatgtgacc ctcgccatgg
     1741 taaatacatg gcttgctgcc tgttgtaccg tggtgacgtg gttcccaaag atgtcaatgc
     1801 tgccattgcc accatcaaaa ccaagcgcag catccagttt gtggattggt gccccactgg
     1861 cttcaaggtt ggcatcaact accagcctcc cactgtggtg cctggtggag acctggccaa
     1921 ggtacagaga gctgtgtgca tgctgagcaa caccacagcc attgctgagg cctgggctcg
     1981 cctggaccac aagtttgacc tgatgtatgc caagcgtgcc tttgttcact ggtacgtggg
     2041 tgaggggatg gaggaaggcg agttttcaga ggcccgtgaa gatatggctg cccttgagaa
     2101 ggattatgag gaggttggtg tggattctgt tgaaggagag ggtgaggaag aaggagagga
     2161 atactaagga tccaccggat ctagataact gatcataatc agccatacca c
//
