LOCUS       sequence-87077-e        2237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 604, Emission: 
            659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1465..1474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1471..2205
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcc catgtccacc
       61 aggtccgtgt cctcgtcctc ctaccgcagg atgttcggcg gcccgggcac cgcgagccgg
      121 ccgagctcca gccggagcta cgtgactacg tccacccgca cctacagcct gggcagcgcg
      181 ctgcgcccca gcaccagccg cagcctctac gcctcgtccc cgggcggcgt gtatgccacg
      241 cgctcctctg ccgtgcgcct gcggagcagc gtgcccgggg tgcggctcct gcaggactcg
      301 gtggacttct cgctggccga cgccatcaac accgagttca agaacacccg caccaacgag
      361 aaggtggagc tgcaggagct gaatgaccgc ttcgccaact acatcgacaa ggtgcgcttc
      421 ctggagcagc agaataagat cctgctggcc gagctcgagc agctcaaggg ccaaggcaag
      481 tcgcgcctgg gggacctcta cgaggaggag atgcgggagc tgcgccggca ggtggaccag
      541 ctaaccaacg acaaagcccg cgtcgaggtg gagcgcgaca acctggccga ggacatcatg
      601 cgcctccggg agaaattgca ggaggagatg cttcagagag aggaagccga aaacaccctg
      661 caatctttca gacaggatgt tgacaatgcg tctctggcac gtcttgacct tgaacgcaaa
      721 gtggaatctt tgcaagaaga gattgccttt ttgaagaaac tccacgaaga ggaaatccag
      781 gagctgcagg ctcagattca ggaacagcat gtccaaatcg atgtggatgt ttccaagcct
      841 gacctcacgg ctgccctgcg tgacgtacgt cagcaatatg aaagtgtggc tgccaagaac
      901 ctgcaggagg cagaagaatg gtacaaatcc aagtttgctg acctctctga ggctgccaac
      961 cggaacaatg acgccctgcg ccaggcaaag caggagtcca ctgagtaccg gagacaggtg
     1021 cagtccctca cctgtgaagt ggatgccctt aaaggaacca atgagtccct ggaacgccag
     1081 atgcgtgaaa tggaagagaa ctttgccgtt gaagctgcta actaccaaga cactattggc
     1141 cgcctgcagg atgagattca gaatatgaag gaggaaatgg ctcgtcacct tcgtgaatac
     1201 caagacctgc tcaatgttaa gatggccctt gacattgaga ttgccaccta caggaagctg
     1261 ctggaaggcg aggagagcag gatttctctg cctcttccaa acttttcctc cctgaacctg
     1321 agggaaacta atctggattc actccctctg gttgataccc actcaaaaag gacacttctg
     1381 attaagacgg ttgaaactag agatggacag gttatcaacg aaacttctca gcatcacgat
     1441 gaccttgaag gggatccacc ggtcgccacc atggtgagca agggcgagga gctgatcaag
     1501 gagaacatgc acatgaagct gtacatggaa ggcaccgtga acaaccacca cttcaagtgc
     1561 accaccgaag gggagggcaa gccctacgag ggcacccaga cccagaggat taaggtggtg
     1621 gagggaggcc ccctgccgtt cgcattcgac atcctggcca cctgctttat gtacgggagc
     1681 aagaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag
     1741 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgcttac cgttacccag
     1801 gacaccagcc tccaggacgg ctgcttgatc tacaacgtca agctcagagg ggtgaacttc
     1861 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccac caccgagacc
     1921 ctgtaccccg ctgacggcgg cctggaaggc agatgcgaca tggccctgaa gctcgtgggc
     1981 gggggccacc tgcactgcaa cctgaagacc acatacagat ccaagaaacc cgctaagaac
     2041 ctcaagatgc ccggcgtcta ctttgtggac cgcagactgg aaagaatcaa ggaggccgac
     2101 aatgagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
     2161 aaactggggc acaaacttaa tggcatggac gagctgtaca agtaagcggc cgcgactcta
     2221 gatcataatc agccata
//
