LOCUS       sequence-87078-e        6056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..759
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     RBS             2460..2468
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gatcaaggag
       61 aacatgcaca tgaagctgta catggaaggc accgtgaaca accaccactt caagtgcacc
      121 accgaagggg agggcaagcc ctacgagggc acccagaccc agaggattaa ggtggtggag
      181 ggaggccccc tgccgttcgc attcgacatc ctggccacct gctttatgta cgggagcaag
      241 accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc
      301 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgcttaccgt tacccaggac
      361 accagcctcc aggacggctg cttgatctac aacgtcaagc tcagaggggt gaacttccca
      421 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaccac cgagaccctg
      481 taccccgctg acggcggcct ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg
      541 ggccacctgc actgcaacct gaagaccaca tacagatcca agaaacccgc taagaacctc
      601 aagatgcccg gcgtctactt tgtggaccgc agactggaaa gaatcaagga ggccgacaat
      661 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
      721 ctggggcaca aacttaatgg catggacgag ctgtacaagt ccggaagtgc tgggagcgca
      781 agcggaagcg gaggctccgg tgaggaaaca gctatatggg aacaacatac agtgacgctt
      841 cacagggctc ctggatttgg atttggaatt gcaatatctg gtggacgaga taatcctcat
      901 tttcagagtg gggaaacgtc aatagtgatt tcagatgtgc tgaaaggagg accagctgaa
      961 ggacagctac aggaaaatga ccgagttgca atggttaacg gagtttcaat ggataatgtt
     1021 gaacatgctt ttgctgttca gcaactaagg aaaagtggga aaaatgcaaa aattacaatt
     1081 agaaggaaga agaaagttca aataccagta agtcgtcctg atcctgaacc agtatctgat
     1141 aatgaagaag atagttatga tgaggaaata catgatccaa gaagtggccg gagtggtgtg
     1201 gttaacagaa ggagtgagaa gatttggccg agggatagaa gtgcaagtag agagaggagc
     1261 ttgtccccgc ggtcagacag gcggtcagtg gcttccagcc agcctgctaa acctactaaa
     1321 gtcacactgg tgaaatcccg gaaaaatgaa gaatatggtc ttcgattggc aagccatata
     1381 tttgttaagg aaatttcaca agatagtttg gcagcaagag atggcaatat tcaagaaggt
     1441 gatgttgtat tgaagataaa tggtactgtg acagaaaata tgtcattgac agatgcaaag
     1501 acattgatag aaaggtctaa aggcaaatta aaaatggtag ttcaaagaga tgaacgggct
     1561 acgctattga atgtccctga tctttctgac agcatccact ctgctaatgc ctctgagaga
     1621 gacgacattt cagaaattca gtcactggca tcagatcatt ctggtcgatc acacgatagg
     1681 cctccccgcc gcagccggtc acgatctcct gaccagcggt cagagccttc tgatcattcc
     1741 aggcactcgc cgcagcagcc aagcaatggc agtctgcgga gtagagatga agagagaatt
     1801 tctaaacctg gggctgtctc aactcctgta aagcatgctg atgatcacac acctaaaaca
     1861 gtggaagaag ttacagttga aagaaatgag aaacaaacac cttctcttcc agaaccaaag
     1921 cctgtgtatg cccaagttgg caaccagatg tggatttacc tgtcagtcca tctgatggtg
     1981 tcctacctaa ttcaactcat gaagatggga tttcttcggc ccagcatgaa attggtaaaa
     2041 ttcagaaaag gagatagtgt gggtttgcgg ctggctggtg gaaatgatgt tggaatattt
     2101 gtagctggcg ttctagaaga tagccctgca gccaaggaag gcttagagga aggtgatcaa
     2161 attctcaggg taaacaacgt agattttaca aatatcataa gagaagaagc cgtccttttc
     2221 ctgcttgacc tccctaaagg agaagaagtg accatattgg ctcagaagaa gaaggatgtt
     2281 tatcgtcgca ttgtagaatc agatgtagga gattctttct atattagaac ccattttgaa
     2341 tatgaaaagg aatctcccta tggacttagt tttaacaaag gagaggtgtt ccgtgctgtg
     2401 gataccttgt acaatggaaa actgggctct tggcttgcta ttcgaattgg taaaaatcat
     2461 aaggaggtag aacgaggcat catccctaat aagaacagag ctgagcagct cgccagtgta
     2521 cagtatacac ttccaaaaac agcaggcgga gaccgtgctg acttctggag attcagaggt
     2581 cttcgcagct ccaagagaaa tcttcgaaaa agcagagagg atttgtccgc tcagcctgtt
     2641 caaacaaagt ttccagctta tgaaagagtg gttcttcgag aagctggatt tctgaggcct
     2701 gtaaccattt ttggaccaat agctgatgtt gccagagaaa agctggcaag agaagaacca
     2761 gatatttatc aaattgcaaa gagtgaacca cgagacgctg gaactgacca acgtagctct
     2821 ggctatattc gcctgcatac aataaagcaa atcatagatc aagacaaaca tgctttatta
     2881 gatgtaacac caaatgcagt tgatcgtctt aactatgccc agtggtatcc aattgttgta
     2941 tttcttaacc ctgattctaa gcaaggagta aaaacaatga gaatgaggtt atgtccagaa
     3001 tctcggaaaa gtgccaggaa gttatacgag cgatctcata aacttgctaa aaataatcac
     3061 catcttttta caactacaat taacttaaat tcaatgaatg atggttggta tggtgcgctg
     3121 aaagaagcag ttcaacaaca gcaaaaccag ctggtatggg tttccgaggg aaaggcggat
     3181 ggtgctacaa gtgatgacct tgatttgcat gatgatcgtc tgtcctacct gtcagctcca
     3241 ggtagtgaat actcaatgta tagcacggac agtagacaca cttctgacta tgaagacaca
     3301 gacacagaag gcggggccta cactgatcaa gaactagatg aaactcttaa tgatgaggtt
     3361 gggactccac cggagtctgc cattacacgg tcctctgagc ctgtaagaga ggactcctct
     3421 ggaatgcatc atgaaaacca aacatatcct ccttactcac cacaagcgca gccacaacca
     3481 attcatagaa tagactcccc tggatttaag ccagcctctc aacagaaagc agaagcttca
     3541 tctccagtcc cttacctttc gcctgaaaca aacccagcat catcaacctc tgctgttaat
     3601 cataatgtaa atttaactaa tgtcagactg gaggagccca ccccagctcc ttccacctct
     3661 tactcaccac aagctgattc tttaagaaca ccaagtactg aggcagctca cataatgcta
     3721 agagatcaag aaccatcatt gtcgtcgcat gtagatccaa caaaggtgta tagaaaggac
     3781 ccatatcccg aggaaatgat gaggcagaac catgttttga aacagccagc cgttagtcac
     3841 ccagggcaca ggccagacaa agagcctaat ctgacctatg aaccccaact cccatacgta
     3901 gagaaacaag ccagcagaga cctggagcag cccacataca gatacgagtc ctcaagctat
     3961 acggaccagt tttctcgaaa ctatgaacat cgtctgcgat acgaagatcg cgtccccatg
     4021 tatgaagaac agtggtcata ttatgatgac aaacagccct acccatctcg gccacctttt
     4081 gataatcagc actctcaaga ccttgactcc agacagcatc ccgaagagtc ctcagaacga
     4141 gggtactttc cacgttttga agagccagcc cctctgtctt acgacagcag accacgttac
     4201 gaacaggcac ctagagcatc cgccctgcgg cacgaagagc agccagctcc tgggtatgac
     4261 acacatggta gactcagacc ggaagcccag ccccaccctt cagcagggcc caagcctgca
     4321 gagtccaagc agtattttga gcaatattca cgcagttacg agcaagtacc accccaagga
     4381 tttacctcta gagcaggtca ttttgagcct ctccatggtg ctgcagctgt ccctccgctg
     4441 ataccttcat ctcagcataa gccagaagct ctgccttcaa acaccaaacc actgcctcca
     4501 cccccaactc aaaccgaaga agaggaagat ccagcaatga agccacagtc tgtactcacc
     4561 agagttaaga tgtttgaaaa caaaaggtct gcatccttag agaccaagaa ggatgtaaat
     4621 gacactggca gttttaagcc tccagaagta gcatctaaac cttcaggtgc tcccatcatt
     4681 ggtcccaaac ccacttctca gaatcaattc agtgaacatg acaaaactct gtacaggatt
     4741 ccagaacctc aaaaacctca actgaagcca cctgaagata ttgttcggtc caatcattat
     4801 gaccctgaag aagatgaaga atattatcga aaacagctgt catactttga ccgaagaagt
     4861 tttgagaata agcctcctgc acacattgct gccagccatc tctccgagcc tgcaaagcca
     4921 gctcattctc agaatcaatc aaatttttct agttattctt caaagggaaa gcctcctgaa
     4981 gctgatggtg tggatagatc atttggcgag aaacgctatg aacccatcca ggccactccc
     5041 cctcctcctc cattgccctc gcagtatgcc cagccatctc agcctgtcac cagcgcgtct
     5101 ctccacatac attctaaggg agcacatggt gaaggtaatt cagtgtcatt ggattttcag
     5161 aattccttag tgtccaaacc agacccacct ccatctcaga ataagccagc aactttcaga
     5221 ccaccaaacc gagaagatac tgctcaggca gctttctatc cccagaaaag ttttccagat
     5281 aaagccccag ttaatggaac tgaacagact cagaaaacag tcactccagc atacaatcga
     5341 ttcacaccaa aaccatatac aagttctgcc cgaccatttg aacgcaagtt tgaaagtcct
     5401 aaattcaatc acaatcttct gccaagtgaa actgcacata aacctgactt gtcttcaaaa
     5461 actcccactt ctccaaaaac tcttgtgaaa tcgcacagtt tggcacagcc tcctgagttt
     5521 gacagtggag ttgaaacttt ctctatccat gcagagaagc ctaaatatca aataaataat
     5581 atcagcacag tgcctaaagc tattcctgtg agtccttcag ctgtggaaga ggatgaagat
     5641 gaagatggtc atactgtggt ggccacagcc cgaggcatat ttaacagcaa tgggggcgtg
     5701 ctgagttcca tagaaactgg tgttagtata attatccctc aaggagccat tcccgaagga
     5761 gttgagcagg aaatctattt caaggtctgc cgggacaaca gcatccttcc acctttagat
     5821 aaagagaaag gtgaaacact gctgagtcct ttggtgatgt gtggtcccca tggcctcaag
     5881 ttcctgaagc ctgtggagct gcgcttacca cactgtgatc ctaaaacctg gcaaaacaag
     5941 tgtcttcccg gagatccaaa ttatctcgtt ggagcaaact gtgtttctgt ccttattgac
     6001 cacttttaag gatccaccgg atctagataa ctgatcataa tcagccatac cacatt
//
