LOCUS       sequence-87089-e        1232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 588, Emission: 615.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1232)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCrimson2-H2B-N-22
FEATURES             Location/Qualifiers
     source          1..1232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..398
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     misc_feature    399..451
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      459..468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcagatccgc tagcgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg
       61 gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc
      121 gcaaggagag ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg
      181 gcatttcgtc caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca
      241 tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg
      301 agatccagac ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg
      361 agggtactaa ggccatcacc aagtacacca gcgctaagct cgagctcaag cttcgaattc
      421 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      481 aggagctgat caaggagaac atgcacagca agctgtacct ggaaggcagc gtgaacggcc
      541 accagttcaa gtgcacccac gaaggggagg gcaagcccta cgagggcacc cagaccaaca
      601 ggatcaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg gccaccatgt
      661 ttatgtacgg gagcaaggcc ttcatcaagt accccaaggg cctccccgat tattttaagc
      721 agtccttccc tgagggcttc acatgggaga gaaccatggt gttcgaagac gggggcgtgc
      781 tgaccgccac ccaggacacc agcctccagg acggctgcct catctacaac gtgaagctga
      841 gaggggtgaa cttcccagcc aacggccccg tgatgaagca gacaacactg ggctgggagc
      901 ccagcaccga gaccctgtac cccgctgacg gcgccctgga aggcagatgc gacatggccc
      961 tgaagctcgt gggcgggggc cacctgcact gcaacttcaa gaccacatac aaatccaaga
     1021 aacccgctac aaacctcaag atgcccggcg tccactacgt ggaccgcaga ctggaaagaa
     1081 tcaaggaggc cgacaacgag acctacgtcg agcagcacga ggtggctgtg gccagatact
     1141 gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg tacaagtaag
     1201 cggccgcgac tctagatcat aatcagccat ac
//
