LOCUS       sequence-87114-e        1592 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1592
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    530..582
                     /label=MCS
                     /note="multiple cloning site"
     CDS             596..1555
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
ORIGIN
        1 gacgtcagat ccgctagcca ccatgggccc caagcggcgg cagctgacgt tccgggagaa
       61 gtcgcggatc atccaggagg tggaggagaa cccggaccta cgcaagggcg agatcgcgcg
      121 gcgcttcaac atcccgccgt ccacgctgag cacgatcctg aagaacaagc gcgccatcct
      181 ggcgtcggag cgcaagtacg gggtggcctc cacctgccgc aagaccaaca agctgtctcc
      241 ctacgacaag ctcgagggct tgctcatcgc ctggttccag cagatccgcg ccgccggcct
      301 gccggtcaag ggcatcatcc tcaaggagaa ggcgctgcgc atagccgagg agctgggcat
      361 ggacgacttc accgcctcca acggctggct ggaccgcttc cgccggcgcc acggcgtggt
      421 gtcctgcagc ggcgtggccc gcgcccgcgc gcgaaacgct gccccccgca ccccggcggc
      481 gcctgccagt ccggccgcgg tgccctcgga gggcagtggc gggagcactc tcgagctcaa
      541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatgtc
      601 ggtaccgctg actacctcag cattcggcca cgcgtttctg gctaactgtg aacgcgagca
      661 gatccacctg gcgggctcca ttcagccgca cggtatcctg ctggctgtga aagagccgga
      721 caacgtggtg atccaggctt ctattaacgc tgcggagttc ctgaacacca actctgttgt
      781 tggccgtccg ctgcgtgacc tgggcggcga tctgcctttg cagatcctgc cgcacctgaa
      841 cggcccgctg cacctggctc cgatgaccct gcgttgtacc gtgggttctc cgccgcgtcg
      901 tgtggactgt accattcatc gtccgtctaa cggcggcctg atcgtagaac tggaaccagc
      961 aaccaagacc actaacattg cgccggctct ggacggtgcg tttcatcgta tcacttcttc
     1021 atcctccctg atgggcctgt gtgacgaaac cgcgactatt atccgtgaga ttactggcta
     1081 cgaccgtgtg atggtagtac gtttcgatga agagggtaat ggcgaaattc tgtccgaacg
     1141 tcgtcgtgcg gacctggaag cgttcctggg taaccgctac ccggcgtcta ctattccgca
     1201 gatcgctcgt cgcctgtacg aacataaccg tgttcgcctg ctggtagatg tgaactatac
     1261 tccggttccg ctacagccgc gcatcagccc gctgaacggt cgtgatctgg atatgtccct
     1321 gtcttgcctg cgctctatgt ccccgatcca ccagaaatac atgcaggaca tgggcgttgg
     1381 cgcgaccctg gtttgctctc tgatggtgtc tggtcgtctg tggggtctga tcgcttgcca
     1441 ccactacgaa ccgcgcttcg ttccgttcca cattcgcgct gctggcgaag cgctggcgga
     1501 aacttgtgcg atccgcatcg cgacgctgga gagctttgca cagtctcagt ccaaatgagc
     1561 ggccgcgact ctagatcata atcagccata cc
//
