LOCUS       sequence-87122-e        1477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-H2B-6
FEATURES             Location/Qualifiers
     source          1..1477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             475..1434
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatgtcg
      481 gtaccgctga ctacctcagc attcggccac gcgtttctgg ctaactgtga acgcgagcag
      541 atccacctgg cgggctccat tcagccgcac ggtatcctgc tggctgtgaa agagccggac
      601 aacgtggtga tccaggcttc tattaacgct gcggagttcc tgaacaccaa ctctgttgtt
      661 ggccgtccgc tgcgtgacct gggcggcgat ctgcctttgc agatcctgcc gcacctgaac
      721 ggcccgctgc acctggctcc gatgaccctg cgttgtaccg tgggttctcc gccgcgtcgt
      781 gtggactgta ccattcatcg tccgtctaac ggcggcctga tcgtagaact ggaaccagca
      841 accaagacca ctaacattgc gccggctctg gacggtgcgt ttcatcgtat cacttcttca
      901 tcctccctga tgggcctgtg tgacgaaacc gcgactatta tccgtgagat tactggctac
      961 gaccgtgtga tggtagtacg tttcgatgaa gagggtaatg gcgaaattct gtccgaacgt
     1021 cgtcgtgcgg acctggaagc gttcctgggt aaccgctacc cggcgtctac tattccgcag
     1081 atcgctcgtc gcctgtacga acataaccgt gttcgcctgc tggtagatgt gaactatact
     1141 ccggttccgc tacagccgcg catcagcccg ctgaacggtc gtgatctgga tatgtccctg
     1201 tcttgcctgc gctctatgtc cccgatccac cagaaataca tgcaggacat gggcgttggc
     1261 gcgaccctgg tttgctctct gatggtgtct ggtcgtctgt ggggtctgat cgcttgccac
     1321 cactacgaac cgcgcttcgt tccgttccac attcgcgctg ctggcgaagc gctggcggaa
     1381 acttgtgcga tccgcatcgc gacgctggag agctttgcac agtctcagtc caaatgagcg
     1441 gccgcgactc tagatcataa tcagccatac cacattg
//
