LOCUS       sequence-87133-e        2439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 683, Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..2439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1458..2417
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
ORIGIN
        1 tcagatccgc tagcgatgcc accatggcca aaagaaatgc cgagaaggaa ctgacagata
       61 ggaattggga tcaagaagat gaagctgaag aggtgggaac attctccatg gccagtgagg
      121 aagtcttgaa gaatagagcc ataaagaaag caaagcgcag aaatgttgga tttgaatctg
      181 acactggagg agcctttaaa ggttttaaag gtttggtggt accttctgga ggaggacgct
      241 tttctggatt tggtagtggc gctggaggga agcctctgga aggactgtcg aatggaaaca
      301 acataaccag tgcccctccc ttcgccagtg caaaggcagc ggcagatccc aaggtagcct
      361 ttggttctct tgctgcaaat ggccctacca ccttggttga taaagtttca aatcccaaaa
      421 ctaatgggga cagtcagcag ccctcctcct ctggccttgc ttccagtaaa gcttgtgtcg
      481 gaaatgccta tcacaagcag ttggccgcct tgaactgctc cgtgcgggat tggatagtga
      541 agcacgtgaa tacaaacccc ctctgtgatc tgacacctat ctttaaagac tatgagaaat
      601 atttagcaaa cattgaacag caacacggga acagtggcag gaattctgaa agtgaatcta
      661 acaaagtggc agctgaaaca cagtctcctt ccctttttgg ctcaacaaaa ttacagcaag
      721 agtcaacgtt tttgtttcat ggcaacaaaa ctgaagatac acctgacaag aagatggagg
      781 tggcatctga aaagaaaacg gacccatcat cactaggagc gacaagtgcc tcatttaatt
      841 tcggcaagaa agttgatagc tctgttttgg gctcattaag ctctgtcccc ctgactggat
      901 tttctttctc ccctggaaac tccagtttat ttggcaaaga tactacccag agtaaaccag
      961 tctcttcacc atttcccact aaaccattgg agggccaagc agaaggtgac agtggtgaat
     1021 gcaaaggtgg agatgaagaa gagaatgatg agccacccaa agtagtagtt accgaagtaa
     1081 aagaagaaga cgctttttac tccaaaaagt gtaaactgtt ttacaagaaa gacaatgagt
     1141 ttaaagagaa aggcataggt actctgcatt taaaacctac agcaaatcag aagacacagc
     1201 ttttggtgcg ggcagacacc aatttaggca acatattgct gaacgttctg attccaccca
     1261 atatgccatg tacgcgaaca gggaagaata acgttcttat cgtctgtgtt ccaaatccac
     1321 caattgacga gaagaatgcc accatgccag tcaccatgtt gattcgggta aaaaccagcg
     1381 aggatgcaga cgagttgcac aaaattttac tggagaaaaa ggatgccagc tccggagggg
     1441 atccaccggt cgccaccatg tcggtaccgc tgactacctc agcattcggc cacgcgtttc
     1501 tggctaactg tgaacgcgag cagatccacc tggcgggctc cattcagccg cacggtatcc
     1561 tgctggctgt gaaagagccg gacaacgtgg tgatccaggc ttctattaac gctgcggagt
     1621 tcctgaacac caactctgtt gttggccgtc cgctgcgtga cctgggcggc gatctgcctt
     1681 tgcagatcct gccgcacctg aacggcccgc tgcacctggc tccgatgacc ctgcgttgta
     1741 ccgtgggttc tccgccgcgt cgtgtggact gtaccattca tcgtccgtct aacggcggcc
     1801 tgatcgtaga actggaacca gcaaccaaga ccactaacat tgcgccggct ctggacggtg
     1861 cgtttcatcg tatcacttct tcatcctccc tgatgggcct gtgtgacgaa accgcgacta
     1921 ttatccgtga gattactggc tacgaccgtg tgatggtagt acgtttcgat gaagagggta
     1981 atggcgaaat tctgtccgaa cgtcgtcgtg cggacctgga agcgttcctg ggtaaccgct
     2041 acccggcgtc tactattccg cagatcgctc gtcgcctgta cgaacataac cgtgttcgcc
     2101 tgctggtaga tgtgaactat actccggttc cgctacagcc gcgcatcagc ccgctgaacg
     2161 gtcgtgatct ggatatgtcc ctgtcttgcc tgcgctctat gtccccgatc caccagaaat
     2221 acatgcagga catgggcgtt ggcgcgaccc tggtttgctc tctgatggtg tctggtcgtc
     2281 tgtggggtct gatcgcttgc caccactacg aaccgcgctt cgttccgttc cacattcgcg
     2341 ctgctggcga agcgctggcg gaaacttgtg cgatccgcat cgcgacgctg gagagctttg
     2401 cacagtctca gtccaaatga gcggccgcga ctctagatc
//
