LOCUS       sequence-87146-e        3829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 683, 
            Emission: 704.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: mIFP12-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..993
                     /codon_start=1
                     /product="naturally monomeric infrared fluorescent protein 
                     derived from a truncated bacteriophytochrome (Yu et al., 
                     2015)"
                     /label=mIFP
                     /translation="MSVPLTTSAFGHAFLANCEREQIHLAGSIQPHGILLAVKEPDNVV
                     IQASINAAEFLNTNSVVGRPLRDLGGDLPLQILPHLNGPLHLAPMTLRCTVGSPPRRVD
                     CTIHRPSNGGLIVELEPATKTTNIAPALDGAFHRITSSSSLMGLCDETATIIREITGYD
                     RVMVVRFDEEGNGEILSERRRADLEAFLGNRYPASTIPQIARRLYEHNRVRLLVDVNYT
                     PVPLQPRISPLNGRDLDMSLSCLRSMSPIHQKYMQDMGVGATLVCSLMVSGRLWGLIAC
                     HHYEPRFVPFHIRAAGEALAETCAIRIATLESFAQSQSK"
     protein_bind    2848..2859
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatgtcgg taccgctgac tacctcagca
       61 ttcggccacg cgtttctggc taactgtgaa cgcgagcaga tccacctggc gggctccatt
      121 cagccgcacg gtatcctgct ggctgtgaaa gagccggaca acgtggtgat ccaggcttct
      181 attaacgctg cggagttcct gaacaccaac tctgttgttg gccgtccgct gcgtgacctg
      241 ggcggcgatc tgcctttgca gatcctgccg cacctgaacg gcccgctgca cctggctccg
      301 atgaccctgc gttgtaccgt gggttctccg ccgcgtcgtg tggactgtac cattcatcgt
      361 ccgtctaacg gcggcctgat cgtagaactg gaaccagcaa ccaagaccac taacattgcg
      421 ccggctctgg acggtgcgtt tcatcgtatc acttcttcat cctccctgat gggcctgtgt
      481 gacgaaaccg cgactattat ccgtgagatt actggctacg accgtgtgat ggtagtacgt
      541 ttcgatgaag agggtaatgg cgaaattctg tccgaacgtc gtcgtgcgga cctggaagcg
      601 ttcctgggta accgctaccc ggcgtctact attccgcaga tcgctcgtcg cctgtacgaa
      661 cataaccgtg ttcgcctgct ggtagatgtg aactatactc cggttccgct acagccgcgc
      721 atcagcccgc tgaacggtcg tgatctggat atgtccctgt cttgcctgcg ctctatgtcc
      781 ccgatccacc agaaatacat gcaggacatg ggcgttggcg cgaccctggt ttgctctctg
      841 atggtgtctg gtcgtctgtg gggtctgatc gcttgccacc actacgaacc gcgcttcgtt
      901 ccgttccaca ttcgcgctgc tggcgaagcg ctggcggaaa cttgtgcgat ccgcatcgcg
      961 acgctggaga gctttgcaca gtctcagtcc aaaggtggcg gaagcggcgg cggaagcggt
     1021 ggcggaagtt ccggactcag atctggcagc agtggaggtt tggaggagcc ggaggccgcg
     1081 acgcggacag ccgcggccgt ggattgcaaa gacagacccg ggttcccagt gaagaggtta
     1141 atacaagccc gcttgccctt caaacgcctg aaccttgttc ccaaggagaa agttgaggaa
     1201 gacacatcac caaaagcagc tgtggaaagc aaagtcccgg atctacaatt gtctttgggc
     1261 acttttgaaa gccagtgtca cacaggctct cacgtaggct tgagtacaaa gttggtcggt
     1321 ggccagggcc ccattgatag cttcttgagg gcaacaatca agccagtacc gagtgtggtc
     1381 attatcgacc tgacggagaa ctgcagtgac attccagaca gccctgaggg ccacagtgaa
     1441 ctaagtcctg acactgctgg tgtggtcacc acagtggaag gagctgccaa gcagcaggag
     1501 cactctgcag cagagctgtg tctcctagag accccttcag acatcacttg tcacatggaa
     1561 gaggagcctg ggagtccagg agacccaaag aggacgggtg actgtcaggc tggttcacta
     1621 cagagctgtc cggagctgac cccaggttct aggacatgcc ccaccaagga gttatcaagc
     1681 tggagcaagg caggggatct cctatttata gagaaagtgc ctgtggtggt gctagaggac
     1741 atcctggcca ctaagccctc cattgcctct cttcccatga tgtccctgga taggagtgtc
     1801 acttcagaga gtgaaatact ggagtcctgc cccgaggatg actccatact gagccactcc
     1861 tccaccaact cctcttctcc aaccagctct cctgagggac catccacacc cccagaacat
     1921 cggggtggga gaagctcccc ctccacccca gcctgcagag ttgccaagaa ctttgtcaaa
     1981 ggctccacag agaagggcag gagcaaactg cacagagata gagaacagca gagggaagag
     2041 aaggaaaagc tgagggaaga gatacgacga gccaaggagg aggcacggaa gaagaaggag
     2101 gaggagaagg agctgaagga aaaggagcgg agggaaaagc gggagaaaga tgagaaggag
     2161 aaggcggaga agcagcggct aaaggaggaa aaacgcaagg agcggcagga agctctggag
     2221 gctaaactgg aggagaagag gaagaaggag gaagagaagc ggttacggga ggaagagaag
     2281 cggttacggg aagaagagaa gcgcattaag gcagaaaagg cagagatcac tcgattcttc
     2341 cagaaaccga agactccgca ggctcccaag accctggccg gttcctgtgg taagtttgcc
     2401 ccctttgaaa tcaaagaaca catggtgctg gctccacggt gccgagctgc cttggatcaa
     2461 gacctgtgcg atcagctgga ccagctcctg cagcagcaga gtgtggcaag caccttcctc
     2521 agtgacctaa aaagccggct gcccttgaga tcggggccca cccgggtctg tggccatgac
     2581 acagacatca tgaacaggga tgtggtcatt gtggagagca gcaaggtgga cggtgtgtct
     2641 gaaaggaaga agtttggccg catgaagctt ctgcagttct cagagaacca ccggccagca
     2701 tactggggga cgtggaacaa gaagacagcc atcatccgcc cccggaaccc ctgggcccag
     2761 gacaaagacc ttcttgacta tgaggtagac agtgacgatg agtgggaaga ggaggaacct
     2821 ggggagtctt tgtcccacag tgagggggat gaggatgacg atgtggggga agatgaggat
     2881 gaggacgatg gcttctttgt gccccatggg tacttatctg aagatgaagg cgtgactgag
     2941 gagtgtgcag accctgagaa ccacaaagta caccagaagc tgaaggccaa ggagtgggat
     3001 gagctcctag ccaagggcaa gcgctttcgt gtgctgcagc ctgtgcacgt gggatgtgta
     3061 tgggcatcag aggcagccaa ctgcacgagt tccgacttga agctgctgca gcagttcact
     3121 gcatgcctgc tggatgtggc atcccctgat gagccagaac ccggggcctc caggagggaa
     3181 aagagggacc agcatatcct ggcccagttg cttccgctgc tacatggcaa tgtgaatggg
     3241 agcaaggtga tcatccatga attccaggag caatgccgca ggggcctgct gacccttcca
     3301 agtcccaccc cacacctgca gatgcccaac ttggaggatg ctgttgctgt gccatccaag
     3361 gccaggctga agcgccttat ctctgagaac tctgcctatg agaagcggcc caacttccgt
     3421 atgtgctggt atgtgcaccc agaggtgctc aagagctttg gccaggagtg cctgccagtg
     3481 ccctgccagt ggacctacat caccaccatg ccctcagcac ccagggaaga cagtggcagt
     3541 gcttctactg aggggcctgg ccagagcaca cctatgctac tcaagcggaa gccagcggcc
     3601 accatgtgca tcacgcagtt tatgaagaag cggcgctatg atggacaggt tgggtctggg
     3661 gacatggatg gcttccaggc agacacagaa gaggatgaag aggatgacac agactgtatg
     3721 atcatagatg tgccagatgt tgggagtgac gtgtcagaag ctcccatacc tgctcccaca
     3781 ctttgcaagt gaggatccac cggatctaga taactgatca taatcagcc
//
