LOCUS       sequence-87184-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LI D-E mOrange + link c2
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..814
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(857..873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(857..873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(870..892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    881..897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(905..911,912..929,930..935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    950..971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gaatgcatct agatatcgga tcccgggccc gtcgactgca gaggtacggg tctcaaaggg
       61 aggtggagga ggttctggag gcggtggaag tggtggcgga ggtagcgtga gcaagggcga
      121 ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc
      181 tgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggctt
      241 tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      301 gtcccctcag ttcacctacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      361 ctacttcaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      421 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      481 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaaccat
      541 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      601 caagatgagg ctgaagctga aggacggcgg ccactacacc tccgaggtca agaccaccta
      661 caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcggcatca agttggacat
      721 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      781 ctccaccggc ggcatggacg agctgtacaa gtaaaatctg agacctctgc ctgcatgcaa
      841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtc
//
