LOCUS       sequence-87186-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 492, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAzami Green-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      247..256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             253..933
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent 
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             253..933
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
ORIGIN
        1 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg
       61 gactcagatc tggtacccct tgcagcttcc agttgtcagg aagagaaggc tcccatggat
      121 cctgtggtgg tgctggggct ctgtctctcc tgtttgcttc tcctttcact ctggaaacag
      181 agctatgggg gagggaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
      241 ccggtcgcca ccatggtgag cgtgatcaag cccgagatga agatcaagct gtgcatgagg
      301 ggcaccgtga acggccacaa cttcgtgatc gagggcgagg gcaagggcaa cccctacgag
      361 ggcacccaga tcctggacct gaacgtgacc gagggcgccc ccctgccctt cgcctacgac
      421 atcctgacca ccgtgttcca gtacggcaac agggccttca ccaagtaccc cgccgacatc
      481 caggactact tcaagcagac cttccccgag ggctaccact gggagaggag catgacctac
      541 gaggaccagg gcatctgcac cgccaccagc aacatcagca tgaggggcga ctgcttcttc
      601 tacgacatca ggttcgacgg caccaacttc ccccccaacg gccccgtgat gcagaagaag
      661 accctgaagt gggagcccag caccgagaag atgtacgtgg aggacggcgt gctgaagggc
      721 gacgtgaaca tgaggctgct gctggagggc ggcggccact acaggtgcga cttcaagacc
      781 acctacaagg ccaagaagga ggtgaggctg cccgacgccc acaagatcga ccacaggatc
      841 gagatcctga agcacgacaa ggactacaac aaggtgaagc tgtacgagaa cgccgtggcc
      901 aggtactcca tgctgcccag ccaggccaag taagcggccg cgactctaga tcataatcag
      961 ccataccaca tttgtagagg ttttacttgc tttaaaaacc tcccacacct ccccctgacc
     1021 tgaaacataa atgatgcatt gtgtgttact tgtttattgc agctt
//
