LOCUS       sequence-87189-e        1339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1339)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      576..585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(582..584,585..587,588..1301)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(582..584,585..587,588..1298)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(627..648)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(888..907)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1235..1256
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttatgtcg agcaagagag
       61 ccaaggccaa gaccaccaag aagaggcccc agagggctac gtccaatgtc ttcgccatgt
      121 ttgaccagtc ccagatccag gagtttaagg aggccttcaa catgattgat cagaaccgcg
      181 atggcttcat tgataaggag gacctgcacg acatgttggc ctctctgggg aagaacccca
      241 cagacgagta cctggagggc atgatgaacg aggcgccagg gcccatcaac ttcaccatgt
      301 tcctcacaat gtttggggag aagctgaacg gcacagaccc cgaggatgtg atccgcaatg
      361 cctttgcctg ctttgatgag gaggcctcag gcttcatcca cgaggaccac ctgagggagc
      421 tgctcaccac catgggcgac cgattcacgg atgaggaggt ggacgagatg taccgcgagg
      481 cacccattga taagaagggc aacttcaact acgtggagtt cactcgcatc ctcaaacacg
      541 gcgccaagga caaggacgac ctggatccac cggtcgccac catggtgagc aagggcgagg
      601 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      661 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      721 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      781 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      841 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      901 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      961 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1021 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1081 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1141 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1201 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1261 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat
     1321 cataatcagc cataccaca
//
