LOCUS       sequence-87190-e        2485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2485)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-Paxillin-KM-N-10
FEATURES             Location/Qualifiers
     source          1..2485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1732..1741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1738..1740,1741..1743,1744..2457)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1738..1740,1741..1743,1744..2454)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1783..1804)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2044..2063)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2391..2412
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatcc atggacgacc tcgatgcctt actggcagac
       61 ctggaatcca ccacctcgca tatctctaag cgaccggtgt ttctaacaga ggaaacgcct
      121 tactcctacc caactggaaa ccatacgtac caggagattg ctgtgccacc gccggtgccc
      181 ccgccgcctt ccagtgaggc cctaaatggc actgtgattg accccttaga ccagtggcaa
      241 cccagcgtgt ccagatacgg ccaccagcaa cctcagtccc agtctccgat atacagctct
      301 agtgccaaaa gctccagtgc ctctgttcct agagacgggc tcagctcccc ttctccccgt
      361 gccagtgagg aggaacacgt gtacagcttc ccaaacaagc agaagtctgc agaaccatct
      421 ccctcactga ccagcacctc cctgggcagc aacctctcgg aactggacag gcttctcctg
      481 gaactgaatg ctgttcaaca taatcccccc agtggcttct cagcagatga agtcagcaga
      541 agcccatcac tgcccaatgt gaccggacct cactatgtca tcccagagag cagcagctct
      601 gcaggaggga aggctgcacc ccctacaaaa gaaaaaccaa agcggaatgg tgggcgtggg
      661 attgaagatg tgcgtcccag tgtggagagc ctgctggatg agctggagag ctctgtgcca
      721 agcccagtcc ctgcaatcac tgtgagccaa ggggaggtga gcagccccca gcgtgtcaac
      781 gccagtcagc agcagacccg tatctctgct tcttcagcta cacgagaact ggatgagctg
      841 atggcgtccc tctctgactt taagttcatg gcacagggga aagccggcgg cagcagctcc
      901 ccaccatcca ccaccccaaa gcctggcagt cagctggaca ccatgctggg aagtctgcag
      961 tctgacctga acaaactggg ggtagcgacg gtcgccaagg gtgtgtgcgg agcctgcaag
     1021 aagccgattg ctgggcaggt agttacagcc atggggaaga cctggcaccc tgagcacttc
     1081 gtctgcaccc actgccagga ggagattgga tcacggaact tctttgagcg ggatgggcag
     1141 ccctactgtg agaaggacta tcacaacctc ttctctcctc gctgctacta ctgcaatggg
     1201 ccaatccttg ataaagtggt gacggctttg gacaggacgt ggcaccctga acactttttc
     1261 tgtgcccagt gtggagtttt ctttggacct gaaggatttc atgagaagga tggcaaagcc
     1321 tattgccgca aggactactt tgacatgttt gctcccaagt gtggaggctg tgcccgggct
     1381 atcctggaaa attacatttc tgccttaaac accctgtggc accctgagtg ctttgtctgt
     1441 cgggaatgtt tcacaccgtt catcaacggc agcttcttcg agcacgacgg ccagccctac
     1501 tgcgaggtgc attaccacga gcgccgcggc tcgctgtgct ctggctgcca gaagcccatc
     1561 acgggacgct gcatcactgc catgggcaag aaatttcacc ccgaacactt tgtctgtgcc
     1621 ttctgcctca agcagctcaa caaaggaacc ttcaaagaac agaacgacaa gccctactgt
     1681 cagaactgct ttctcaaact cttctgtggt accgcgggcc cgggatccat cgccaccatg
     1741 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     1801 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1861 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     1921 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     1981 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2041 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2101 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2161 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2221 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2281 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2341 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2401 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     2461 ggccgcgact ctagatcata atcag
//
