LOCUS       sequence-87195-e        3053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3053)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEGFP-TPX2-N-10
FEATURES             Location/Qualifiers
     source          1..3053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2291..2300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2297..2299,2300..2302,2303..3016)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2342..2363)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2603..2622)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2950..2971
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatgtc acaagttaaa agctcttatt cctatgatgc
       61 cccctcggat ttcatcaatt tttcatcctt ggatgatgaa ggagatactc aaaacataga
      121 ttcatggttt gaggagaagg ccaatttgga gaataagtta ctggggaaga atggaactgg
      181 agggcttttt cagggcaaaa ctcctttgag aaaggctaat cttcagcaag ctattgtcac
      241 acctttgaaa ccagttgaca acacttacta caaagaggca gaaaaagaaa atcttgtgga
      301 acaatccatt ccgtcaaatg cttgttcttc cctggaagtt gaggcagcca tatcaagaaa
      361 aactccagcc cagcctcaga gaagatctct taggctttct gctcagaagg atttggaaca
      421 gaaagaaaag catcatgtaa aaatgaaagc caagagatgt gccactcctg taatcatcga
      481 tgaaattcta ccctctaaga aaatgaaagt ttctaacaac aaaaagaagc cagaggaaga
      541 aggcagtgct catcaagata ctgctgaaaa gaatgcatct tccccagaga aagccaaggg
      601 tagacatact gtgccttgta tgccacctgc aaagcagaag tttctaaaaa gtactgagga
      661 gcaagagctg gagaagagta tgaaaatgca gcaagaggtg gtggagatgc ggaaaaagaa
      721 tgaagaattc aagaaacttg ctctggctgg aatagggcaa cctgtgaaga aatcagtgag
      781 ccaggtcacc aaatcagttg acttccactt ccgcacagat gagcgaatca aacaacatcc
      841 taagaaccag gaggaatata aggaagtgaa ctttacatct gaactacgaa agcatccttc
      901 atctcctgcc cgagtgacta agggatgtac cattgttaag cctttcaacc tgtcccaagg
      961 aaagaaaaga acatttgatg aaacagtttc tacatatgtg ccccttgcac agcaagttga
     1021 agacttccat aaacgaaccc ctaacagata tcatttgagg agcaagaagg atgatattaa
     1081 cctgttaccc tccaaatctt ctgtgaccaa gatttgcaga gacccacaga ctcctgtact
     1141 gcaaaccaaa caccgtgcac gggctgtgac ctgcaaaagt acagcagagc tggaggctga
     1201 ggagctcgag aaattgcaac aatacaaatt caaagcacgt gaacttgatc ccagaatact
     1261 tgaaggtggg cccatcttgc ccaagaaacc acctgtgaaa ccacccaccg agcctattgg
     1321 ctttgatttg gaaattgaga aaagaatcca ggagcgagaa tcaaagaaga aaacagagga
     1381 tgaacacttt gaatttcatt ccagaccttg ccctactaag attttggaag atgttgtggg
     1441 tgttcctgaa aagaaggtac ttccaatcac cgtccccaag tcaccagcct ttgcattgaa
     1501 gaacagaatt cgaatgccca ccaaagaaga tgaggaagag gacgaaccgg tagtgataaa
     1561 agctcaacct gtgccacatt atggggtgcc ttttaagccc caaatcccag aggcaagaac
     1621 tgtggaaata tgccctttct cgtttgattc tcgagacaaa gaacgtcagt tacagaagga
     1681 gaagaaaata aaagaactgc agaaagggga ggtgcccaag ttcaaggcac ttcccttgcc
     1741 tcattttgac accattaacc tgccagagaa gaaggtaaag aatgtgaccc agattgaacc
     1801 tttctgcttg gagactgaca gaagaggtgc tctgaaggca cagacttgga agcaccagct
     1861 ggaagaagaa ctgagacagc agaaagaagc agcttgtttc aaggctcgtc caaacaccgt
     1921 catctctcag gagccctttg ttcccaagaa agagaagaaa tcagttgctg agggcctttc
     1981 tggttctcta gttcaggaac cttttcagct ggctactgag aagagagcca aagagcggca
     2041 ggagctggag aagagaatgg ctgaggtaga agcccagaaa gcccagcagt tggaggaggc
     2101 cagactacag gaggaagagc agaaaaaaga ggagctggcc aggctacgga gagaactggt
     2161 gcataaggca aatccaatac gcaagtacca gggtctggag ataaagtcaa gtgaccagcc
     2221 tctgactgtg cctgtatctc ccaaattctc cactcgattc cactgctcgg gaagcacgga
     2281 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2341 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2401 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2461 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2521 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2581 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2641 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2701 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     2761 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     2821 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2881 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     2941 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3001 cgagctgtac aagtaaagcg gccgcgactc tagacataat cagccatacc aca
//
