LOCUS       sequence-87199-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..49
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     regulatory      268..277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(274..276,277..279,280..993)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(319..340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     927..948
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgac
       61 cgtcagatcc gctagcgcta ccggactcag atctggtacc cttgcagctt ccagtgtcag
      121 gaagagaagg ctcccatgga tcctgtggtg gtgctggggc tctgtctctc ctgtttgctt
      181 ctcctttcac tctggaaaca gagctatggg ggagggaagc ttcgaattct gcagtcgacg
      241 gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      301 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      361 gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      421 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      481 cagtgcttca gccgctaccc cgaccacatg aagcgccacg acttcttcaa gtccgccatg
      541 cccgaaggct acgtccagga gcgcaccatc agcttcaagg acgacggcac ctacaagacc
      601 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      661 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
      721 aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc
      781 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      841 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc
      901 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      961 atcactcacg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     1021 ccataccaca tttgtagagg ttttacttgc tttaaaaacc tccca
//
