LOCUS       sequence-87202-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 493, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mWasabi-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      223..232
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             229..939
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYK"
ORIGIN
        1 acatgggtag tgacgtcaga tccgctagcg ctaccggact cagatctggt accccttgca
       61 gcttccagtt gtcaggaaga gaaggctccc atggatcctg tggtggtgct ggggctctgt
      121 ctctcctgtt tgcttctcct ttcactctgg aaacagagct atgggggagg gaagcttcga
      181 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
      241 ggcgaggaga ccacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag
      301 ggcaacgtga atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac
      361 ggcaccaaca ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac
      421 attctgacca ccgcgttcag ttacggcaac agggccttca ccaagtaccc cgacgacatc
      481 cccaactact tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc
      541 gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc
      601 tacgagatac acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaaggag
      661 accaccggct gggacgcctc caccgagagg atgtacgtgc gcgacggcgt gctgaagggc
      721 gacgtcaaga tgaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc
      781 atctacaggg ccaagaaggc ggtgaagctg cccgactatc actttgtgga ccaccgcatc
      841 gagatcctga accacgacaa ggactacaac aaggtgaccg tttacgagat cgccgtggcc
      901 cgcaactcca ccgacggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
      961 aatcagccat accacatgta gagatctc
//
