LOCUS       sequence-87207-e        1140 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1140
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             394..1113
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(439..460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1047..1068
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgacgtca gatccgctag cgccaccatg aagttaagtc gccagttcac cgtgtttggc
       61 agcgcgatct tctgcgtcgt aatcttctca ctctacctga tgctggacag gggtcacttg
      121 gactaccctc ggggcccgcg ccaggagggc tcctttccgc agggccagct ttcaatattg
      181 caagaaaaga ttgaccattt ggagcgtttg ctcgctgaga acaacgagat catctcaaat
      241 atcagagact cagtcatcaa cctgagcgag tctgtggagg acggcccgcg ggggtcacca
      301 ggcaacgcca gccaaggctc catccacctc cactcgccac agttggccct gcaggctgac
      361 cccagctccg gatcggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      481 gtccgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg
      601 gcctgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      661 cccgaaggct acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc
      721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
      841 aacgtctata tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc
      901 cacaacgttg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      961 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc cgccctgagc
     1021 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1081 atcactctcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
//
