LOCUS       sequence-87208-e        2208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1451..1460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1457..2176
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1502..1523)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2110..2131
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggaatccgct agcgatgcca ccatggccaa aagaaatgcc gagaaggaac tgacagatag
       61 gaattgggat caagaagatg aagctgaaga ggtgggaaca ttctccatgg ccagtgagga
      121 agtcttgaag aatagagcca taaagaaagc aaagcgcaga aatgttggat ttgaatctga
      181 cactggagga gcctttaaag gttttaaagg tttggtggta ccttctggag gaggacgctt
      241 ttctggattt ggtagtggcg ctggagggaa gcctctggaa ggactgtcga atggaaacaa
      301 cataaccagt gcccctccct tcgccagtgc aaaggcagcg gcagatccca aggtagcctt
      361 tggttctctt gctgcaaatg gccctaccac cttggttgat aaagtttcaa atcccaaaac
      421 taatggggac agtcagcagc cctcctcctc tggccttgct tccagtaaag cttgtgtcgg
      481 aaatgcctat cacaagcagt tggccgcctt gaactgctcc gtgcgggatt ggatagtgaa
      541 gcacgtgaat acaaaccccc tctgtgatct gacacctatc tttaaagact atgagaaata
      601 tttagcaaac attgaacagc aacacgggaa cagtggcagg aattctgaaa gtgaatctaa
      661 caaagtggca gctgaaacac agtctccttc cctttttggc tcaacaaaat tacagcaaga
      721 gtcaacgttt ttgtttcatg gcaacaaaac tgaagataca cctgacaaga agatggaggt
      781 ggcatctgaa aagaaaacgg acccatcatc actaggagcg acaagtgcct catttaattt
      841 cggcaagaaa gttgatagct ctgttttggg ctcattaagc tctgtccccc tgactggatt
      901 ttctttctcc cctggaaact ccagtttatt tggcaaagat actacccaga gtaaaccagt
      961 ctcttcacca tttcccacta aaccattgga gggccaagca gaaggtgaca gtggtgaatg
     1021 caaaggtgga gatgaagaag agaatgatga gccacccaaa gtagtagtta ccgaagtaaa
     1081 agaagaagac gctttttact ccaaaaagtg taaactgttt tacaagaaag acaatgagtt
     1141 taaagagaaa ggcataggta ctctgcattt aaaacctaca gcaaatcaga agacacagct
     1201 tttggtgcgg gcagacacca atttaggcaa catattgctg aacgttctga ttccacccaa
     1261 tatgccatgt acgcgaacag ggaagaataa cgttcttatc gtctgtgttc caaatccacc
     1321 aattgacgag aagaatgcca ccatgccagt caccatgttg attcgggtaa aaaccagcga
     1381 ggatgcagac gagttgcaca aaattttact ggagaaaaag gatgccagct ccggagggga
     1441 tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1501 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga
     1561 gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1621 cgtgccctgg cccaccctcg tgaccacctt cggctacggc gtggcctgct tcagccgcta
     1681 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1741 ggagcgcacc atctctttca aggacgacgg tacctacaag acccgcgccg aggtgaagtt
     1801 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1861 caacatcctg gggcacaagc tggagtacaa cttcaacagc cacaacgtct atatcacggc
     1921 cgacaagcag aagaacggca tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg
     1981 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     2041 gctgcccgac aaccactacc tgagccatca gtccgccctg agcaaagacc ccaacgagaa
     2101 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     2161 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccata
//
