LOCUS       sequence-87209-e        1976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..1976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1222..1231
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1228..1947
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1273..1294)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1881..1902
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cggtcaggat ccgctagcac cgccaccatg aggaagatgc tcgccgccgt ctcccgcgtg
       61 ctgtctggcg cttctcagaa gccggcaagc agagtgctgg tagcatcccg taattttgca
      121 aatgatgcta catttgaaat taagaaatgt gaccttcacc ggctggaaga aggccctcct
      181 gtcacaacag tgctcaccag ggaggatggg ctcaaatact acaggatgat gcagactgta
      241 cgccgaatgg agttgaaagc agatcagctg tataaacaga aaattattcg tggtttctgt
      301 cacttgtgtg atggtcagga agcttgctgt gtgggcctgg aggccggcat caaccccaca
      361 gaccatctca tcacagccta ccgggctcac ggctttactt tcacccgggg cctttccgtc
      421 cgagaaattc tcgcagagct tacaggacga aaaggaggtt gtgctaaagg gaaaggagga
      481 tcgatgcaca tgtatgccaa gaacttctac gggggcaatg gcatcgtggg agcgcaggtg
      541 cccctgggcg ctgggattgc tctagcctgt aagtataatg gaaaagatga ggtctgcctg
      601 actttatatg gcgatggtgc tgctaaccag ggccagatat tcgaagctta caacatggca
      661 gctttgtgga aattaccttg tattttcatc tgtgagaata atcgctatgg aatgggaacg
      721 tctgttgaga gagcggcagc cagcactgat tactacaaga gaggcgattt cattcctggg
      781 ctgagagtgg atggaatgga tatcctgtgc gtccgagagg caacaaggtt tgctgctgcc
      841 tattgtagat ctgggaaggg gcccatcctg atggagctgc agacttaccg ttaccacgga
      901 cacagtatga gtgaccctgg agtcagttac cgtacacgag aagaaattca ggaagtaaga
      961 agtaagagtg accctattat gcttctcaag gacaggatgg tgaacagcaa tcttgccagt
     1021 gtggaagaac taaaggaaat tgatgtggaa gtgaggaagg agattgagga tgctgcccag
     1081 tttgccacgg ccgatcctga gccacctttg gaagagctgg gctaccacat ctactccagc
     1141 gacccacctt ttgaagttcg tggtgccaat cagtggatca agtttaagtc agtcagtagc
     1201 tccggagggg atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1261 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtccgc
     1321 ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat ctgcaccacc
     1381 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cgtggcctgc
     1441 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1501 ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa gacccgcgcc
     1561 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1621 aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag ccacaacgtc
     1681 tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat ccgccacaac
     1741 gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1801 ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct gagcaaagac
     1861 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1921 ctcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa tcagcc
//
