LOCUS       sequence-87211-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      200..209
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             206..925
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(251..272)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     859..880
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggactcagat ctcgagctcg ccaccatgat tcacaccaac ctgaagaaaa agttcagctg
       61 ctgcgtcctg gtctttcttc tgtttgcagt catctgtgtg tggaaggaaa agaagaaagg
      121 gagttactat gattccttta aattgcaaac caaggaattc ctgcagtcga cggtaccgcg
      181 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      241 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtccgcgg
      301 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg
      361 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tggcctgctt
      421 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      481 ctacgtccag gagcgcacca tctctttcaa ggacgacggt acctacaaga cccgcgccga
      541 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      601 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacgtcta
      661 tatcacggcc gacaagcaga agaacggcat caaggctaac ttcaagatcc gccacaacgt
      721 tgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
      781 ccccgtgctg ctgcccgaca accactacct gagccatcag tccgccctga gcaaagaccc
      841 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
      901 cggcatggac gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca
//
