LOCUS       sequence-87212-e        1241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 505, 
            Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      485..494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             491..1210
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(536..557)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1144..1165
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacggtcaga tccgctagcg ccaccatggt gggtcggaac agcgccatcg ccgccggtgt
       61 atgcggggcc cttttcattg ggtactgcat ctacttcgac cgcaaaagac gaagtgaccc
      121 caacttcaag aacaggcttc gagaacgaag aaagaaacag aagcttgcca aggagagagc
      181 tgggctttcc aagttacctg accttaaaga tgctgaagct gttcagaagt tcttccttga
      241 agaaatacag cttggtgaag agttactagc tcaaggtgaa tatgagaagg gcgtagacca
      301 tctgacaaat gcaattgctg tgtgtggaca gccacagcag ttactgcagg tcttacagca
      361 aactcttcca ccaccagtgt tccagatgct tctgactaag ctcccaacaa ttagtcagag
      421 aattgtaagt gctcagagct tggctgaaga tgatgtggaa ggcggtagcg gggatccacc
      481 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      541 cgagctggac ggcgacgtaa acggccacaa gttcagcgtc cgcggcgagg gcgagggcga
      601 tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      661 ctggcccacc ctcgtgacca ccttcggcta cggcgtggcc tgcttcagcc gctaccccga
      721 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      781 caccatctct ttcaaggacg acggtaccta caagacccgc gccgaggtga agttcgaggg
      841 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      901 cctggggcac aagctggagt acaacttcaa cagccacaac gtctatatca cggccgacaa
      961 gcagaagaac ggcatcaagg ctaacttcaa gatccgccac aacgttgagg acggcagcgt
     1021 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     1081 cgacaaccac tacctgagcc atcagtccgc cctgagcaaa gaccccaacg agaagcgcga
     1141 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1201 gtacaagtaa gcggccgcga ctctagatca taatcagcca t
//
