LOCUS       sequence-87213-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515.
            This plasmid encodes dClover2 A206K, H231L based on publication..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: dClover2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      205..214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             211..930
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHYVYITADKQKNSIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(256..277)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     864..885
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctg gtaccccttg cagcttccag ttgtcaggaa
       61 gagaaggctc ccatggatcc tgtggtggtg ctggggctct gtctctcctg tttgcttctc
      121 ctttcactct ggaaacagag ctatggggga gggaagcttc gaattctgca gtcgacggta
      181 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      241 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtc
      301 cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc
      361 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcgtggcc
      421 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      481 gaaggctacg tccaggagcg caccatctct ttcaaggacg acggtaccta caagacccgc
      541 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      601 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacttcaa cagccactac
      661 gtctatatca cggccgacaa gcagaagaac agcatcaagg ctaacttcaa gatccgccac
      721 aacgttgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      781 gacggccccg tgctgctgcc cgacaaccac tacctgagcc atcagtccaa gctgagcaaa
      841 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      901 actctcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca
      961 taccacattt gtagaggttt tacttgcttt aaaaacctcc cacacctccc c
//
