LOCUS       sequence-87215-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      205..214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(211..213,214..216,217..930)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(256..277)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agatccgcta gcgctaccgg actcagatct ggtacccctt gcagcttcca gtgtcaggaa
       61 gagaaggctc ccatggatcc tgtggtggtg ctggggctct gtctctcctg tttgcttctc
      121 ctttcactct ggaaacagag ctatggggga gggaagcttc gaattctgca gtcgacggta
      181 ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      241 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      301 cgcggcgagg gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc
      361 tccggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgtctta cggcgtgcag
      421 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      481 gaaggctacg tccaggagcg caccatctcc ttcaaggacg acggcagcta caggacccgc
      541 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      601 ttcaaggagg acggcaacat cctggggcac aagctggagt acaacatgaa cgtgtgggac
      661 gcgtatatca cggccgacaa gcagaagaac ggcatcaaag cgaacttcaa gatcgagcac
      721 aacgtcgagg acggcggcgt gcagctcgcc gacgcgtacc agcagaacac ccccatcggc
      781 gacggctccg tgctgctgcc tgacaaccac tacctgagct tccagagcaa gctgttcaaa
      841 gaccccaacg agcagcgcga tcacatggtc ctgctggagt tcgttaccgc cgccgggatc
      901 actcccggca tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca
      961 taccacattt gtagaggttt tacttgcttt aaaaacctcc cacacctccc cctgaactga
     1021 aacataaaat gaatgcaatt gttgttgtta acttgtttat gcagc
//
