LOCUS       sequence-87219-e        1320 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1320)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1320
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      569..578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(575..577,578..580,581..1294)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(620..641)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(881..900)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1228..1249
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagct caagcttatg tcgagcaaga gagccaaggc
       61 caagaccacc aagaagaggc cccagagggc tacgtccaat gtcttcgcca tgtttgacca
      121 gtcccagatc caggagttta aggaggcctt caacatgatt gatcagaacc gcgatggctt
      181 cattgataag gaggacctgc acgacatgtt ggcctctctg gggaagaacc ccacagacga
      241 gtacctggag ggcatgatga acgaggcgcc agggcccatc aacttcacca tgttcctcac
      301 aatgtttggg gagaagctga acggcacaga ccccgaggat gtgatccgca atgcctttgc
      361 ctgctttgat gaggaggcct caggcttcat ccacgaggac cacctgaggg agctgctcac
      421 caccatgggc gaccgattca cggatgagga ggtggacgag atgtaccgcg aggcacccat
      481 tgataagaag ggcaacttca actacgtgga gttcactcgc atcctcaaac acggcgccaa
      541 ggacaaggac gacctggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
      601 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      661 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      721 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
      781 gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      841 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      901 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      961 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1021 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1081 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1141 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1201 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1261 gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
//
