LOCUS       sequence-87220-e         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      216..225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(222..224,225..227,228..941)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(267..288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(528..547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     875..896
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcgccac catgattcac accaacctga
       61 agaaaaagtt cagctgctgc gtcctggtct ttcttctgtt tgcagtcatc tgtgtgtgga
      121 aggaaaagaa gaaagggagt tactatgatt cctttaaatt gcaaaccaag gaattcctgc
      181 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
      241 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      301 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      361 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
      421 acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      481 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      541 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      601 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      661 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      721 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      781 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
      841 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      901 ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgcg actctagatc
      961 ataatcagcc ataccacatt t
//
