LOCUS       sequence-87221-e        3166 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mCitrine-VE-Cadherin-N-10
FEATURES             Location/Qualifiers
     source          1..3166
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2070..2081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      2403..2412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2409..2411,2412..2414,2415..3128)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2454..2475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2715..2734)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3062..3083
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaccgtcaga tccgctagcc gccaccatgc agaggctcat gatgctcctc gccacatcgg
       61 gcgcctgcct gggcctgctg gcagtggcag cagtggcagc agcaggtgct aaccctgccc
      121 aacgggacac ccacagcctg ctgcccaccc accggcgcca aaagagagat tggatttgga
      181 accagatgca cattgatgaa gagaaaaaca cctcacttcc ccatcatgta ggcaagatca
      241 agtcaagcgt gagtcgcaag aatgccaagt acctgctcaa aggagaatat gtgggcaagg
      301 tcttccgggt cgatgcagag acaggagacg tgttcgccat tgagaggctg gaccgggaga
      361 atatctcaga gtaccacctc actgctgtca ttgtggacaa ggacactggt gaaaacctgg
      421 agactccttc cagcttcacc atcaaagttc atgacgtgaa cgacaactgg cctgtgttca
      481 cgcatcggtt gttcaatgcg tccgtgcctg agtcgtcggc tgtggggacc tcagtcatct
      541 ctgtgacagc agtggatgca gacgacccca ctgtgggaga ccacgcctct gtcatgtacc
      601 aaatcctgaa ggggaaagag tattttgcca tcgataattc tggacgtatt atcacaataa
      661 cgaaaagctt ggaccgagag aagcaggcca ggtatgagat cgtggtggaa gcgcgagatg
      721 cccagggcct ccggggggac tcgggcacgg ccaccgtgct ggtcactctg caagacatca
      781 atgacaactt ccccttcttc acccagacca agtacacatt tgtcgtgcct gaagacaccc
      841 gtgtgggcac ctctgtgggc tctctgtttg ttgaggaccc agatgagccc cagaaccgga
      901 tgaccaagta cagcatcttg cggggcgact accaggacgc tttcaccatt gagacaaacc
      961 ccgcccacaa cgagggcatc atcaagccca tgaagcctct ggattatgaa tacatccagc
     1021 aatacagctt catcgtcgag gccacagacc ccaccatcga cctccgatac atgagccctc
     1081 ccgcgggaaa cagagcccag gtcattatca acatcacaga tgtggacgag ccccccattt
     1141 tccagcagcc tttctaccac ttccagctga aggaaaacca gaagaagcct ctgattggca
     1201 cagtgctggc catggaccct gatgcggcta ggcatagcat tggatactcc atccgcagga
     1261 ccagtgacaa gggccagttc ttccgagtca caaaaaaggg ggacatttac aatgagaaag
     1321 aactggacag agaagtctac ccctggtata acctgactgt ggaggccaaa gaactggatt
     1381 ccactggaac ccccacagga aaagaatcca ttgtgcaagt ccacattgaa gttttggatg
     1441 agaatgacaa tgccccggag tttgccaagc cctaccagcc caaagtgtgt gagaacgctg
     1501 tccatggcca gctggtcctg cagatctccg caacagacaa ggacataaca ccacgaaacg
     1561 tgaagttcaa attcactttg aatactgaga acaactttac cctcacggat aatcacgata
     1621 acacggccaa catcacagtc aagtatgggc agtttgaccg ggagcatacc aaggtccact
     1681 tcctacccgt ggtcatctca gacaatggga tgccaagtcg cacgggcacc agcacgctga
     1741 ccgtggccgt gtgcaagtgc aacgagcagg gcgagttcac cttctgcgag gatatggccg
     1801 cccaggtggg cgtgagcatc caggcagtgg tagccatctt actctgcatc ctcaccatca
     1861 cagtgatcac cctgctcatc ttcctgcggc ggcggctccg gaagcaggcc cgcgcgcacg
     1921 gcaagagcgt gccggagatc cacgagcagc tggtcaccta cgacgaggag ggcggcggcg
     1981 agatggacac caccagctac gatgtgtcgg tgctcaactc ggtgcgccgc ggcggggcca
     2041 agcccccgcg gcccgcgctg gacgcccggc cttccctcta tgcgcaggtg cagaagccac
     2101 cgaggcacgc gcctggggca cacggagggc ccggggagat ggcagccatg atcgaggtga
     2161 agaaggacga ggcggaccac gacggcgacg gcccccccta cgacacgctg cacatctacg
     2221 gctacgaggg ctccgagtcc atagccgagt ccctcagctc cctgggcacc gactcatccg
     2281 actctgacgt ggattacgac ttccttaacg actggggacc caggtttaag atgctggctg
     2341 agctgtacgg ctcggacccc cgggaggagc tgctgtattc gggaagcacg gatccaccgg
     2401 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2461 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2521 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2581 ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc
     2641 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2701 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2761 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2821 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2881 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2941 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3001 acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
     3061 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3121 acaagtaagc ggccgcgact ctagatcata atcagccata ccacat
//
