LOCUS       sequence-87223-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Venus-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      269..278
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(275..277,278..280,281..994)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(320..341)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(581..600)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     928..949
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgcccatgac gcatgcgtag gcgggtacgg tgggaggtct atttaagcag agctgtttag
       61 ggacgtcaga tccgctagcc gctacgactc agatctggta cccttgcagc ttccagttgt
      121 cagagagaag gctcccatgg atcctgtggt ggtgctgggg ctctgtctct cctgtttgct
      181 tctcctttca ctctggaaac agagctatgg gggagggaag cttcgaattc tgcagtcgac
      241 ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt
      301 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      361 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      421 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
      481 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      541 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      601 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      661 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
      721 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
      781 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
      841 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
      901 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      961 gatcactctc ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gatcatatca
     1021 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcc
//
