LOCUS       sequence-87224-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-ATG16-N-18
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1859..1868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1865..1867,1868..1870,1871..2584)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1910..1931)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2171..2190)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2518..2539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggact cagatctcga gctcaagctt accgccatgt cgtcgggcct
       61 gcgcgccgca gactttcccc gctggaagcg tcacatcgcg gaggaactga ggcgccggga
      121 ccgactgcag aggcaggcgt tcgaggagat cattctgcag tataccaagt tgctggaaaa
      181 gtcagatctt cattcagtat tgacccagaa actacaagca gaaaagcatg acatgccaaa
      241 taggcatgaa ataagtcctg gacatgatgg tgcgtggaat gatagtcaac tacaagaaat
      301 ggcccagttg aggatcaaac accaggaaga gctgaccgaa ctgcacaaga agcgtgggga
      361 gttagctcag ttggtgattg acctgaacaa ccaaatgcag cagaaggaca aggagataca
      421 gatgaatgaa gcaaagattt cggagtattt acagaccatc tctgacctgg agacaaactg
      481 cctggacctg cgcaccaaac tgcaggacct cgaggtagcc aaccagaccc tgaaggatga
      541 gtatgacgcc ctgcagatta cttttactgc cctagaagag aaactgagga aaactactga
      601 ggagaaccag gaactggtca ccagatggat ggctgagaag gcccaagaag ccaatcgcct
      661 caatgcagag aatgagaagg actccaggag gcgtcaagca cggctgcaga aggagcttgc
      721 agaagcagca aaggaacctc tacctgttga acaggatgat gacattgaag tcattgtgga
      781 tgagacctca gaccacacag aagagacctc tcccgtccga gctgtcagca gagcagctac
      841 gagacgctct gtctcttcca tcccagtccc ccaggatatc atggacactc atcctgcttc
      901 tggtaaagat gtgagagtcc caactactgc ctcgtatgtc ttcgatgcgc atgacggaga
      961 ggtcaacgca gtgcagttca gtccaggctc ccggttgctg gccactggag gcatggaccg
     1021 cagggtgaaa ctttgggaag cattcggaga taaatgtgaa ttcaagggct ccctgtctgg
     1081 cagtaatgct ggaattacaa gcattgaatt tgatagtgct ggagcttacc tattagcagc
     1141 ttcaaatgat tttgcaagcc gaatctggac tgtggatgat tatcgattac ggcacacact
     1201 cacaggccac agcgggaaag tcctctctgc caagttcctg ctggacaatg cacggattgt
     1261 ctcaggaagt cacgaccgga ccctcaaact ctgggatctc cgcagcaaag tctgcataaa
     1321 aacagtgttt gcaggatcca gctgcaatga cattgtttgc actgaacaat gtgtaatgag
     1381 tggacatttt gacaagaaaa ttcgtttctg ggatatccgg tcagagagtg tggtccgaga
     1441 gatggaactg ttagggaaga tcactgctct ggacctaaac cctgagagaa ctgagctcct
     1501 gagctgctcc cgtgatgacc tgctaaaagt catcgacctc cggacaaatg cagtcaaaca
     1561 gacattcagt gcgcctggat tcaaatgcgg ctctgactgg acccgggttg tcttcagccc
     1621 tgatggcagt tacgtggcag caggctcagc cgagggttct ctttatgtct ggagtgtgct
     1681 gacagggaaa gtggagaagg ttctttcaaa acagcacagc tcttctatca atgcggtggc
     1741 gtgggccccc tcgggcttac atgttgtcag tgtggacaaa ggaagcagag ctatgctgtg
     1801 ggcacagcct gggagaggca gcgggagtgg cagtcgcgga ggcagtggtg gaccggtcgc
     1861 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1921 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1981 ctacggcaag ctgaccctga agctgatctg caccaccggc aagctgcccg tgccctggcc
     2041 caccctcgtg accaccctgg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
     2101 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2161 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2221 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2281 gcacaagctg gagtacaact acaacagcca caacgtctat atcaccgccg acaagcagaa
     2341 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     2401 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2461 ccactacctg agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     2521 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2581 gtaaagcggc cgcgactcta gatcataatc agccatacca catttgt
//
