LOCUS       sequence-87225-e        2579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2579)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1834..1843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1840..1842,1843..1845,1846..2559)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1885..1906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2146..2165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2493..2514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gactcagatc tcgagaccgc catggaaggg aagtggttgc tgtgtatgtt actggtgctt
       61 ggaactgcta ttgttgaggc tcatgatgga catgatgatg atgtgattga tattgaggat
      121 gaccttgacg atgtcattga agaggtagaa gactcaaaac cagataccac tgctcctcct
      181 tcatctccca aggttactta caaagctcca gttccaacag gggaagtata ttttgctgat
      241 tcttttgaca gaggaactct gtcagggtgg attttatcca aagccaagaa agacgatacc
      301 gatgatgaaa ttgccaaata tgatggaaag tgggaggtag aggaaatgaa ggagtcaaag
      361 cttccaggtg ataaaggact tgtgttgatg tctcgggcca agcatcatgc catctctgct
      421 aaactgaaca agcccttcct gtttgacacc aagcctctca ttgttcagta tgaggttaat
      481 ttccaaaatg gaatagaatg tggtggtgcc tatgtgaaac tgctttctaa aacaccagaa
      541 ctcaacctgg atcagttcca tgacaagacc ccttatacga ttatgtttgg tccagataaa
      601 tgtggagagg actataaact gcacttcatc ttccgacaca aaaaccccaa aacgggtatc
      661 tatgaagaaa aacatgctaa gaggccagat gcagatctga agacctattt tactgataag
      721 aaaacacatc tttacacact aatcttgaat ccagataata gttttgaaat actggttgac
      781 caatctgtgg tgaatagtgg aaatctgctc aatgacatga ctcctcctgt aaatccttca
      841 cgtgaaattg aggacccaga agaccggaag cccgaggatt gggatgaaag accaaaaatc
      901 ccagatccag aagctgtcaa gccagatgac tgggatgaag atgcccctgc taagattcca
      961 gatgaagagg ccacaaaacc cgaaggctgg ttagatgatg agcctgagta cgtacctgat
     1021 ccagacgcag agaaacctga ggattgggat gaagacatgg atggagaatg ggaggctcct
     1081 cagattgcca accctagatg tgagtcagct cctggatgtg gtgtctggca gcgacctgtg
     1141 attgacaacc ccaattataa aggcaaatgg aagcctccta tgattgacaa tcccagttac
     1201 cagggaatct ggaaacccag gaaaatacca aatccagatt tctttgaaga tctggaacct
     1261 ttcagaatga ctccttttag tgctattggt ttggagctgt ggtccatgac ctctgacatt
     1321 ttttttgaca actttatcat ttgtgctgat cgaagaatag ttgatgattg ggccaatgat
     1381 ggatggggcc tgaagaaagc tgctgatggg gctgctgagc caggcgttgt ggggcagatg
     1441 aacgaggcag ctgaagagcg cccgtggctg tgggtagtct atattctaac tgtagccctt
     1501 cctgtgttcc tggttatcct cttctgctgt tctggaaaga aacagaccag tggtatggag
     1561 tataagaaaa ctgatgcacc tcaaccggat gtgaaggaag aggaagaaga gaaggaagag
     1621 gaaaaggaca agggagatga ggaggaggaa ggagaagaga aacttgaaga gaaacagaaa
     1681 agtgatgctg aagaagatgg tggcactgtc agtcaagagg aggaagacag aaaacctaaa
     1741 gcagaggagg atgaaatttt gaacagatca ccaagaaaca gaaagccacg aagagagggg
     1801 ggtgcgtcag gcggcagcgg atctggaccg gtcgccacca tggtgagcaa gggcgaggag
     1861 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1921 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg
     1981 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac
     2041 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     2101 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     2161 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     2221 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     2281 agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     2341 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     2401 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag
     2461 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     2521 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatc
//
