LOCUS       sequence-87226-e        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mVenus-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    531..583
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      591..600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(597..599,600..602,603..1316)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(642..663)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(903..922)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1250..1271
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacggtcaga tccgctagcc accatgggcc ccaagcggcg gcagctgacg ttccgggaga
       61 agtcgcggat catccaggag gtggaggaga acccggacct acgcaagggc gagatcgcgc
      121 ggcgcttcaa catcccgccg tccacgctga gcacgatcct gaagaacaag cgcgccatcc
      181 tggcgtcgga gcgcaagtac ggggtggcct ccacctgccg caagaccaac aagctgtctc
      241 cctacgacaa gctcgagggc ttgctcatcg cctggttcca gcagatccgc gccgccggcc
      301 tgccggtcaa gggcatcatc ctcaaggaga aggcgctgcg catagccgag gagctgggca
      361 tggacgactt caccgcctcc aacggctggc tggaccgctt ccgccggcgc cacggcgtgg
      421 tgtcctgcag cggcgtggcc cgcgcccgcg cgcgaaacgc tgccccccgc accccggcgg
      481 cgcctgccag tccggccgcg gtgccctcgg agggcagtgg cgggagcact ctcgagctca
      541 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg
      601 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      661 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      721 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      781 tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
      841 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      901 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      961 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1021 tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
     1081 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     1141 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1201 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1261 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg
     1321 gccgcgactc tagatcataa tcagccata
//
