LOCUS       sequence-87227-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      216..225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(222..224,225..227,228..941)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(267..288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(528..547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     875..896
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tggtacccct tgcagcttcc
       61 agtgtcagga agagaaggct cccatggatc ctgtggtggt gctggggctc tgtctctcct
      121 gtttgcttct cctttcactc tggaaacaga gctatggggg agggaagctt cgaattctgc
      181 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg
      241 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      301 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
      361 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
      421 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      481 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      541 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      601 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      661 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      721 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
      781 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
      841 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      901 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat
      961 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacactc
     1021 cccctgacct gaaacataaa tgatgcatgt gtgtacttgt ttatt
//
