LOCUS       sequence-8723-ex        7076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7076)
  TITLE     Knockdown Rescue Plasmid
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pResQ shFEN3 3XF-FEN1 wt
FEATURES             Location/Qualifiers
     source          1..7076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..160
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             203..383
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    430..555
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1048..1281
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1466..1510
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1808..1925
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1978..2226
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1978..1998
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2149..2168
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2297..3508
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3345..3364
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     complement(4753..4773)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4758..5087
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4938..5073
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5097..5696
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5097..5116)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5593..5613
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5801..6389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5854..5874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6272..6283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6392..6408)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6393..6409)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
       61 cattgacgtc aatgggagtt tgtttggcac caaaatcaac gggactttcc aaaatgtcgt
      121 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      181 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      241 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      301 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      361 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      421 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      481 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      541 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      601 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
      661 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
      721 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
      781 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
      841 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
      901 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
      961 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1021 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1081 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1141 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1201 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1261 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1321 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1381 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1441 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1501 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1561 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1621 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     1681 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     1741 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     1801 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1861 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1921 attttcgggt ttattacagg gacagcagag atccagtttg gttaattcta gaattctgag
     1981 ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt tagagagata
     2041 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
     2101 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
     2161 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2221 aacaccggtt aagagctaca gctagagaat tcagattctc tagctgtagc tcttaatttt
     2281 tctcgagatc tcaattggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     2341 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     2401 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     2461 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     2521 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     2581 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     2641 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     2701 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     2761 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     2821 ggggttgggg ggagcgcagc aaaatggcgg ctgttcycga gtcttgaatg gaagacgctt
     2881 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     2941 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     3001 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     3061 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     3121 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     3181 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     3241 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     3301 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     3361 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     3421 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     3481 ctggccgttt ttggcttttt tgttagacgg aattcacgcg tatggactac aaagaccatg
     3541 acggtgatta taaagatcat gacatcgatt acaaggatga cgatgactta attaaaggaa
     3601 ttcaaggcct ggccaaacta attgctgatg tggcccccag tgccatccgg gagaatgaca
     3661 tcaagagcta ctttggccgt aaggtggcca ttgatgcctc tatgagcatt tatcagttcc
     3721 tgattgctgt tcgccagggt ggggatgtgc tgcagaatga ggagggtgag accaccagcc
     3781 acctgatggg catgttctac cgcaccattc gcatgatgga gaacggcatc aagcccgtgt
     3841 atgtctttga tggcaagccg ccacagctca agtcaggcga gctggccaaa cgcagtgagc
     3901 ggcgggctga ggcagagaag cagctgcagc aggctcaggc tgctggggcc gagcaggagg
     3961 tggaaaaatt cactaagcgg ctggtgaagg tcactaagca gcacaatgat gagtgcaaac
     4021 atctgctgag cctcatgggc atcccttatc ttgatgcacc cagtgaggca gaggccagct
     4081 gtgctgccct ggtgaaggct ggcaaagtct atgctgcggc taccgaggac atggactgcc
     4141 tcaccttcgg cagccctgtg ctaatgcgac acctgactgc cagtgaagcc aaaaagctgc
     4201 caatccagga attccacctg agccggattc tgcaggagct gggcctgaac caggaacagt
     4261 ttgtggatct gtgcatcctg ctaggcagtg actactgtga gagtatccgg ggtattgggc
     4321 ccaagcgggc tgtggacctc atccagaagc acaagagcat cgaggagatc gtgcggcgac
     4381 ttgaccccaa caagtaccct gtgccagaaa attggctcca caaggaggct caccagctct
     4441 tcttggaacc tgaggtgctg gacccagagt ctgtggagct gaagtggagc gagccaaatg
     4501 aagaagagct gatcaagttc atgtgtggtg aaaagcagtt ctctgaggag cgaatccgca
     4561 gtggggtcaa gaggctgagt aagagccgcc aaggcagcac ccagggccgc ctggatgatt
     4621 tcttcaaggt gaccggctca ctctcttcag ctaagcgcaa ggagccagaa cccaagggat
     4681 ccactaagaa gaaggcaaag actggggcag cagggaagtt taaaagggga aaataagttt
     4741 aaacacagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4801 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4861 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4921 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4981 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5041 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttaccatga
     5101 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     5161 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     5221 acatcgagcg ggtcaccgag ctacaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     5281 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     5341 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     5401 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     5461 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     5521 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5581 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5641 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     5701 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg acccatcgtc gacgcggccg
     5761 cgactctagt ccctcccaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     5821 aatttgtgaa agattgactg gwattcttaa ctatgttgct ccttttacgc tatgtggata
     5881 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5941 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6001 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6061 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6121 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6181 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     6241 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6301 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     6361 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag
     6421 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6481 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6541 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6601 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6661 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     6721 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     6781 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     6841 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     6901 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttag
//
