LOCUS       sequence-87231-e        3251 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Integrin/Focal Adhesions, Excitation: 515, Emission: 
            528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3251)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Integrin-Beta1-N-18
FEATURES             Location/Qualifiers
     source          1..3251
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2480..2489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2486..2488,2489..2491,2492..3205)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2531..2552)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2792..2811)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3139..3160
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tccgctagcg ctaccggact cagatctcga gaccgccatg aatttacaac caattttctg
       61 gattggactg atcagttcag tttgctgtgt gtttgctcaa acagatgaaa atagatgttt
      121 aaaagcaaat gccaaatcat gtggagaatg tatacaagca gggccaaatt gtgggtggtg
      181 cacaaattca acatttttac aggaaggaat gcctacttct gcacgatgtg atgatttaga
      241 agccttaaaa aagaagggtt gccctccaga tgacatagaa aatcccagag gctccaaaga
      301 tataaagaaa aataaaaatg taaccaaccg tagcaaagga acagcagaga agctcaagcc
      361 agaggatatt actcagatcc aaccacagca gttggttttg cgattaagat caggggagcc
      421 acagacattt acattaaaat tcaagagagc tgaagactat cccattgacc tctactacct
      481 tatggacctg tcttactcaa tgaaagacga tttggagaat gtaaaaagtc ttggaacaga
      541 tctgatgaat gaaatgagga ggattacttc ggacttcaga attggatttg gctcatttgt
      601 ggaaaagact gtgatgcctt acattagcac aacaccagct aagctcagga acccttgcac
      661 aagtgaacag aactgcacca gcccatttag ctacaaaaat gtgctcagtc ttactaataa
      721 aggagaagta tttaatgaac ttgttggaaa acagcgcata tctggaaatt tggattctcc
      781 agaaggtggt ttcgatgcca tcatgcaagt tgcagtttgt ggatcactga ttggctggag
      841 gaatgttaca cggctgctgg tgttttccac agatgccggg tttcactttg ctggagatgg
      901 gaaacttggt ggcattgttt taccaaatga tggacaatgt cacctggaaa ataatatgta
      961 cacaatgagc cattattatg attatccttc tattgctcac cttgtccaga aactgagtga
     1021 aaataatatt cagacaattt ttgcagttac tgaagaattt cagcctgttt acaaggagct
     1081 gaaaaacttg atccctaagt cagcagtagg aacattatct gcaaattcta gcaatgtaat
     1141 tcagttgatc attgatgcat acaattccct ttcctcagaa gtcattttgg aaaacggcaa
     1201 attgtcagaa ggagtaacaa taagttacaa atcttactgc aagaacgggg tgaatggaac
     1261 aggggaaaat ggaagaaaat gttccaatat ttccattgga gatgaggttc aatttgaaat
     1321 tagcataact tcaaataagt gtccaaaaaa ggattctgac agctttaaaa ttaggcctct
     1381 gggctttacg gaggaagtag aggttattct tcagtacatc tgtgaatgtg aatgccaaag
     1441 cgaaggcatc cctgaaagtc ccaagtgtca tgaaggaaat gggacatttg agtgtggcgc
     1501 gtgcaggtgc aatgaagggc gtgttggtag acattgtgaa tgcagcacag atgaagttaa
     1561 cagtgaagac atggatgctt actgcaggaa agaaaacagt tcagaaatct gcagtaacaa
     1621 tggagagtgc gtctgcggac agtgtgtttg taggaagagg gataatacaa atgaaattta
     1681 ttctggcaaa ttctgcgagt gtgataattt caactgtgat agatccaatg gcttaatttg
     1741 tggaggaaat ggtgtttgca agtgtcgtgt gtgtgagtgc aaccccaact acactggcag
     1801 tgcatgtgac tgttctttgg atactagtac ttgtgaagcc agcaacggac agatctgcaa
     1861 tggccggggc atctgcgagt gtggtgtctg taagtgtaca gatccgaagt ttcaagggca
     1921 aacgtgtgag atgtgtcaga cctgccttgg tgtctgtgct gagcataaag aatgtgttca
     1981 gtgcagagcc ttcaataaag gagaaaagaa agacacatgc acacaggaat gttcctattt
     2041 taacattacc aaggtagaaa gtcgggacaa attaccccag ccggtccaac ctgatcctgt
     2101 gtcccattgt aaggagaagg atgttgacga ctgttggttc tattttacgt attcagtgaa
     2161 tgggaacaac gaggtcatgg ttcatgttgt ggagaatcca gagtgtccca ctggtccaga
     2221 catcattcca attgtagctg gtgtggttgc tggaattgtt cttattggcc ttgcattact
     2281 gctgatatgg aagcttttaa tgataattca tgacagaagg gagtttgcta aatttgaaaa
     2341 ggagaaaatg aatgccaaat gggacacggg tgaaaatcct atttataaga gtgccgtaac
     2401 aactgtggtc aatccgaagt atgagggaaa aggtagcgca tccgggggtg cgtcaggcgg
     2461 cagcggatct ggaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2521 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     2581 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
     2641 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt
     2701 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     2761 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     2821 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     2881 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     2941 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     3001 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3061 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     3121 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3181 cggcatggac gagctgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc
     3241 acattgtaga g
//
