LOCUS       sequence-87232-e        1330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1330)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      565..574
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(571..573,574..576,577..1290)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(616..637)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(877..896)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1224..1245
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gcgctaccgg actcagatct cgagctcaag cttatgtcga gcaagagagc caaggccaag
       61 accaccaaga agaggcccca gagggctacg tccaatgtct tcgccatgtt tgaccagtcc
      121 cagatccagg agtttaagga ggccttcaac atgattgatc agaaccgcga tggcttcatt
      181 gataaggagg acctgcacga catgttggcc tctctgggga agaaccccac agacgagtac
      241 ctggagggca tgatgaacga ggcgccaggg cccatcaact tcaccatgtt cctcacaatg
      301 tttggggaga agctgaacgg cacagacccc gaggatgtga tccgcaatgc ctttgcctgc
      361 tttgatgagg aggcctcagg cttcatccac gaggaccacc tgagggagct gctcaccacc
      421 atgggcgacc gattcacgga tgaggaggtg gacgagatgt accgcgaggc acccattgat
      481 aagaagggca acttcaacta cgtggagttc actcgcatcc tcaaacacgg cgccaaggac
      541 aaggacgacc tggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
      601 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      661 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
      721 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag
      781 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      841 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      901 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      961 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1021 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1081 aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1141 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa
     1201 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1261 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     1321 ataccacatt
//
