LOCUS       sequence-87233-e        1152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1152)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..1152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      386..395
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(392..394,395..397,398..1111)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(437..458)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(698..717)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1045..1066
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacggtcaga tccgctagcg ccaccatgaa gttaagtcgc cagttcaccg tgtttggcag
       61 cgcgatcttc tgcgtcgtaa tcttctcact ctacctgatg ctggacaggg gtcacttgga
      121 ctaccctcgg ggcccgcgcc aggagggctc ctttccgcag ggccagcttt caatattgca
      181 agaaaagatt gaccatttgg agcgtttgct cgctgagaac aacgagatca tctcaaatat
      241 cagagactca gtcatcaacc tgagcgagtc tgtggaggac ggcccgcggg ggtcaccagg
      301 caacgccagc caaggctcca tccacctcca ctcgccacag ttggccctgc aggctgaccc
      361 cagctccgga tcggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac
      421 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      481 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac
      541 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca
      601 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      661 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      721 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
      781 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
      841 cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca
      901 caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
      961 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa
     1021 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1081 cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc
     1141 cataccacat tt
//
