LOCUS       sequence-87235-e        2518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2518)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Paxillin-KM-N-18
FEATURES             Location/Qualifiers
     source          1..2518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1753..1762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1759..1761,1762..1764,1765..2478)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1804..1825)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2065..2084)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2412..2433
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgatg gacgacctcg atgccttact ggcagacctg
       61 gaatccacca cctcgcatat ctctaagcga ccggtgtttc taacagagga aacgccttac
      121 tcctacccaa ctggaaacca tacgtaccag gagattgctg tgccaccgcc ggtgcccccg
      181 ccgccttcca gtgaggccct aaatggcact gtgattgacc ccttagacca gtggcaaccc
      241 agcgtgtcca gatacggcca ccagcaacct cagtcccagt ctccgatata cagctctagt
      301 gccaaaagct ccagtgcctc tgttcctaga gacgggctca gctccccttc tccccgtgcc
      361 agtgaggagg aacacgtgta cagcttccca aacaagcaga agtctgcaga accatctccc
      421 tcactgacca gcacctccct gggcagcaac ctctcggaac tggacaggct tctcctggaa
      481 ctgaatgctg ttcaacataa tccccccagt ggcttctcag cagatgaagt cagcagaagc
      541 ccatcactgc ccaatgtgac cggacctcac tatgtcatcc cagagagcag cagctctgca
      601 ggagggaagg ctgcaccccc tacaaaagaa aaaccaaagc ggaatggtgg gcgtgggatt
      661 gaagatgtgc gtcccagtgt ggagagcctg ctggatgagc tggagagctc tgtgccaagc
      721 ccagtccctg caatcactgt gagccaaggg gaggtgagca gcccccagcg tgtcaacgcc
      781 agtcagcagc agacccgtat ctctgcttct tcagctacac gagaactgga tgagctgatg
      841 gcgtccctct ctgactttaa gttcatggca caggggaaag ccggcggcag cagctcccca
      901 ccatccacca ccccaaagcc tggcagtcag ctggacacca tgctgggaag tctgcagtct
      961 gacctgaaca aactgggggt agcgacggtc gccaagggtg tgtgcggagc ctgcaagaag
     1021 ccgattgctg ggcaggtagt tacagccatg gggaagacct ggcaccctga gcacttcgtc
     1081 tgcacccact gccaggagga gattggatca cggaacttct ttgagcggga tgggcagccc
     1141 tactgtgaga aggactatca caacctcttc tctcctcgct gctactactg caatgggcca
     1201 atccttgata aagtggtgac ggctttggac aggacgtggc accctgaaca ctttttctgt
     1261 gcccagtgtg gagttttctt tggacctgaa ggatttcatg agaaggatgg caaagcctat
     1321 tgccgcaagg actactttga catgtttgct cccaagtgtg gaggctgtgc ccgggctatc
     1381 ctggaaaatt acatttctgc cttaaacacc ctgtggcacc ctgagtgctt tgtctgtcgg
     1441 gaatgtttca caccgttcat caacggcagc ttcttcgagc acgacggcca gccctactgc
     1501 gaggtgcatt accacgagcg ccgcggctcg ctgtgctctg gctgccagaa gcccatcacg
     1561 ggacgctgca tcactgccat gggcaagaaa tttcaccccg aacactttgt ctgtgccttc
     1621 tgcctcaagc agctcaacaa aggaaccttc aaagaacaga acgacaagcc ctactgtcag
     1681 aactgctttc tcaaactctt ctgtagcagc ggtggagcaa gcgcagccag tggtagcgcg
     1741 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1801 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1861 gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac cggcaagctg
     1921 cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcctgcagtg cttcgcccgc
     1981 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2041 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2101 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2161 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcacc
     2221 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     2281 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2341 ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga ccccaacgag
     2401 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2461 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatt
//
