LOCUS       sequence-87236-e        1912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1912)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Sequestosome1-N-18
FEATURES             Location/Qualifiers
     source          1..1912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1142..1151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1148..1150,1151..1153,1154..1867)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1193..1214)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1454..1473)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1801..1822
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgacgtcaga tccgctagcg ccaccatggc catgtcctac gtgaaggatg acatcttccg
       61 aatctacatt aaagagaaaa aagagtgccg gcgggaccac cgcccaccgt gtgctcagga
      121 ggcgccccgc aacatggtgc accccaatgt gatctgcgat ggctgcaatg ggcctgtggt
      181 aggaacccgc tacaagtgca gcgtctgccc agactacgac ttgtgtagcg tctgcgaggg
      241 aaagggcttg caccgggggc acaccaagct cgcattcccc agccccttcg ggcacctgtc
      301 tgagggcttc tcgcacagcc gctggctccg gaaggtgaaa cacggacact tcgggtggcc
      361 aggatgggaa atgggtccac caggaaactg gagcccacgt cctcctcgtg caggggaggc
      421 ccgccctggc cccacggcag aatcagcttc tggtccatcg gaggatccga gtgtgaattt
      481 cctgaagaac gttggggaga gtgtggcagc tgcccttagc cctctgggca ttgaagttga
      541 tatcgatgtg gagcacggag ggaaaagaag ccgcctgacc cccgtctctc cagagagttc
      601 cagcacagag gagaagagca gctcacagcc aagcagctgc tgctctgatc ccagcaagcc
      661 gggtgggaat gttgagggcg ccacgcagtc tctggcggag cagatgagaa agatcgcctt
      721 ggagtccgag gggcgccctg aggaacagat ggagtcggat aactgttcag gaggagatga
      781 tgactggacc catctgtctt caaaagaagt ggacccgtct acaggtgaac tccagtccct
      841 acagatgcca gaatccgaag ggccaagctc tctggacccc tcccaggagg gacccacagg
      901 gctgaaggaa gctgccttgt acccacatct cccgccagag gctgacccgc ggctgattga
      961 gtccctctcc cagatgctgt ccatgggctt ctctgatgaa ggcggctggc tcaccaggct
     1021 cctgcagacc aagaactatg acatcggagc ggctctggac accatccagt attcaaagca
     1081 tcccccgccg ttgagcagcg gtggagcaag cgcagccagt ggtagcgcag cgggaccggt
     1141 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1201 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1261 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     1321 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     1381 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1441 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1501 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1561 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca
     1621 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     1681 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1741 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     1801 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1861 caagtaaagc ggccgcgact ctagatcata atcagccata ccacattgta ga
//
