LOCUS       sequence-87237-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        20..71
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     22..42
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      305..314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(311..313,314..316,317..1030)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(356..377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(617..636)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     964..985
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
       61 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
      121 gctcgccacc atgattcaca ccaacctgaa gaaaaagttc agctgctgcg tcctggtctt
      181 tcttctgttt gcagtcatct gtgtgtggaa ggaaaagaag aaagggagtt actatgattc
      241 ctttaaattg caaaccaagg aattcctgca gtcgacggta ccgcgggccc gggatccacc
      301 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      361 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      421 tgccacctac ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc
      481 ctggcccacc ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga
      541 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      601 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      661 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      721 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa
      781 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
      841 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      901 cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg agaagcgcga
      961 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     1021 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     1081 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaa
//
